Petti L, Sample J, Wang F, Kieff E
Kovler Viral Oncology Laboratory, University of Chicago, Illinois 60637.
J Virol. 1988 Apr;62(4):1330-8. doi: 10.1128/JVI.62.4.1330-1338.1988.
Three distantly homologous neighboring long open reading frames in the Epstein-Barr virus (EBV) genome are preceded by short open reading frames. The leftmost short and long open reading frames encode EBNA3, a nuclear protein which is slightly smaller (145 kilodaltons [kDa]) than two other nuclear proteins (150 to 155 kDa) detected in Western blots (immunoblots) of latently infected cell protein (K. Hennessy, F. Wang, E. Woodland-Bushman, and E. Kieff, Proc. Natl. Acad. Sci. USA 83:5693-5697, 1986; I. Joab, D. T. Rowe, M. Bodescot, J.-C. Nicolas, P. J. Farrell, and M. Perricaudet, J. Virol. 61:3340-3344, 1987). We have demonstrated that the most rightward short (BERF3) and long (BERF4) open reading frames are spliced in frame at the 3' end of a 5-kilobase latently infected cell RNA and that this RNA begins within or upstream of the EBV long internal repeat. EBV-immune human antibodies specific for the long open reading frame translation product identified a 155-kDa protein on Western blots of latently infected cell protein and specifically reacted with large nonnucleolar nuclear granules in every latently infected cell. Expression of the cDNA in BALB/c 3T3 cells resulted in translation of full-size EBNA3C but had no effect on cell morphology, contact inhibition, or serum independence.
爱泼斯坦-巴尔病毒（EBV）基因组中有三个相距较远的同源相邻长开放阅读框，其前面是短开放阅读框。最左边的短开放阅读框和长开放阅读框编码EBNA3，一种核蛋白，其分子量（145千道尔顿[kDa]）略小于在潜伏感染细胞蛋白的蛋白质免疫印迹（免疫印迹）中检测到的另外两种核蛋白（150至155 kDa）（K. Hennessy、F. Wang、E. Woodland-Bushman和E. Kieff，《美国国家科学院院刊》83:5693 - 5697，1986年；I. Joab、D. T. Rowe、M. Bodescot、J.-C. Nicolas、P. J. Farrell和M. Perricaudet，《病毒学杂志》61:3340 - 3344，1987年）。我们已经证明，最右边的短开放阅读框（BERF3）和长开放阅读框（BERF4）在一个5千碱基的潜伏感染细胞RNA的3'端进行框内剪接，并且该RNA起始于EBV长内部重复序列内或其上游。对长开放阅读框翻译产物具有特异性的EBV免疫人抗体在潜伏感染细胞蛋白的蛋白质免疫印迹上鉴定出一种155-kDa的蛋白质，并与每个潜伏感染细胞中的大型非核仁核颗粒发生特异性反应。该cDNA在BALB/c 3T3细胞中的表达导致全长EBNA3C的翻译，但对细胞形态、接触抑制或血清非依赖性没有影响。
