Kubašová Ivana, Strompfová Viola, Lauková Andrea
Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia.
Folia Microbiol (Praha). 2017 Nov;62(6):491-498. doi: 10.1007/s12223-017-0521-z. Epub 2017 Mar 18.
Enterococci form a complex, diverse, and very important group of bacteria from the technological and food safety aspect, or from the health-improving aspect as probiotics. Generally, enterococci are considered to be of low pathogenic potential, which is associated mostly with clinical strains. In these strains, production of virulence factors as well as resistance to many antimicrobial drugs could complicate treatment of nosocomial infections. Because there is a lack of information on incidence of these attributes in animal commensal enterococci, we screened 160 strains originating from feces of clinically healthy dogs in Eastern Slovakia (n = 105). The predominant species were Enterococcus faecium (57.5%) followed by Enterococcus faecalis (21.9%), and Enterococcus hirae (17.5%), while Enterococcus casseliflavus (1.9%) and Enterococcus mundtii (1.2%) rarely occurred. Among the tested antibiotics, gentamicin (high level) was the most effective drug against canine enterococci (95% of isolates were sensitive). In contrast, the highest resistance recorded (71.9%) was to teicoplanin. PCR screening showed the highest incidence of virulence genes in E. faecalis species. The most frequently detected were genes encoding adhesins efa and efa and sex pheromone cpd. IS16 gene, a marker specific for hospital strains, appeared in nine E. faecium strains. No strain was positive for DNase activity, 8.8% of the isolated strains showed gelatinase activity, and almost 100% strains produced tyramine. It seems commensal-derived enterococci from dogs could also to some extent be potential reservoir of risk factors for other microbiota or organisms.
从技术和食品安全方面，或者从作为益生菌改善健康的角度来看，肠球菌是一类复杂、多样且非常重要的细菌。一般来说，肠球菌被认为致病潜力较低，这主要与临床菌株有关。在这些菌株中，毒力因子的产生以及对许多抗菌药物的耐药性会使医院感染的治疗变得复杂。由于缺乏关于动物共生肠球菌中这些特性发生率的信息，我们对斯洛伐克东部临床健康犬粪便中的160株菌株进行了筛选（n = 105）。优势菌种为屎肠球菌（57.5%），其次是粪肠球菌（21.9%）和平肠球菌（17.5%），而格氏肠球菌（1.9%）和蒙氏肠球菌（1.2%）很少出现。在所测试的抗生素中，庆大霉素（高浓度）是对抗犬肠球菌最有效的药物（95%的分离株敏感）。相比之下，记录到的最高耐药率（71.9%）是对替考拉宁。PCR筛选显示粪肠球菌种中毒力基因的发生率最高。最常检测到的是编码黏附素efa 和efa 以及性信息素cpd的基因。IS16基因是医院菌株的特异性标志物，在9株屎肠球菌菌株中出现。没有菌株的DNase活性呈阳性，8.8%的分离菌株显示明胶酶活性，几乎100%的菌株产生酪胺。似乎来自犬的共生源肠球菌在某种程度上也可能是其他微生物群或生物体危险因素的潜在储存库。
