Liu Fengping, Ling Zongxin, Xiao Yonghong, Yang Qing, Wang Baohong, Zheng Li, Jiang Ping, Li Lanjuan, Wang Wei
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang UniversityHangzhou, China; Nursing School, Yancheng Medical CollegeYancheng, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang UniversityHangzhou, China; Department of Urology, First Affiliated Hospital, School of Medicine, Zhejiang UniversityHangzhou, China.
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang UniversityHangzhou, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang UniversityHangzhou, China.
Front Physiol. 2017 Mar 3;8:126. doi: 10.3389/fphys.2017.00126. eCollection 2017.
Evidence shows urine specimens from different women have different populations of bacteria. The co-occurrence of hypertension and hyperlipidemia in those with diabetes may alter the composition of urine and the microenviroment of the bladder in which bacteria live. The aim of this study was to characterize the urinary microbiota in women with type 2 diabetes mellitus only and those with diabetes plus hypertension and/or hyperlipidemia, and to explore whether the composition of the urinary microbiota is affected by fasting blood glucose, blood pressure, and blood lipids. We enrolled 28 individuals with diabetes only, 24 with diabetes plus hypertension, 7 with diabetes plus hyperlipidemia, and 11 with diabetes plus both hypertension and hyperlipidemia. Modified midstream urine collection technique was designed to obtain urine specimens. Bacterial genomic DNA was isolated using magnetic beads and the urinary microbiota was analyzed using the Illumina MiSeq Sequencing System based on the V3-V4 hypervariable regions of the 16S rRNA gene. Among the four cohorts, the diabetes plus hypertension cohort had the highest relative abundance of Proteobacteria. In contrast, the diabetes plus hyperlipidemia cohort had the lowest relative abundance of Proteobacteria. In addition, and were not found in the diabetes plus hyperlipidemia cohort but they were found in all of the other cohorts. was only present in the diabetes plus hypertension cohort. The most abundant bacteria in the diabetes only and diabetes plus hyperlipidemia cohorts was , while was the most abundant bacteria in the diabetes plus hypertension and diabetes plus hypertension and hyperlipidemia cohorts. Moreover, the relative abundance of was significantly lower in the diabetes plus hypertension cohort than in the diabetes only and diabetes plus hyperlipidemia cohorts. Several bacteria were correlated with the participants' fasting blood glucose, blood pressure, and blood lipids. In conclusion, hypertension and/or hyperlipidemia and other patient factors can affect the composition of the urinary microbiota in those with diabetes. The insights from this study could be used to develop microbiota-based treatment for comorbid conditions, including urinary tract infections, in those with diabetes.
证据表明，不同女性的尿液标本中细菌种类不同。糖尿病患者中高血压和高脂血症的共存可能会改变尿液成分以及细菌生存的膀胱微环境。本研究的目的是描述仅患有2型糖尿病的女性以及患有糖尿病合并高血压和/或高脂血症的女性的尿液微生物群特征，并探讨尿液微生物群的组成是否受空腹血糖、血压和血脂的影响。我们招募了28名单纯糖尿病患者、24名糖尿病合并高血压患者、7名糖尿病合并高脂血症患者以及11名糖尿病合并高血压和高脂血症患者。采用改良的中段尿采集技术获取尿液标本。使用磁珠分离细菌基因组DNA，并基于16S rRNA基因的V3-V4高变区，利用Illumina MiSeq测序系统分析尿液微生物群。在这四个队列中，糖尿病合并高血压队列中变形菌门的相对丰度最高。相比之下，糖尿病合并高脂血症队列中变形菌门的相对丰度最低。此外，[此处原文缺失两种细菌名称]在糖尿病合并高脂血症队列中未发现，但在所有其他队列中均有发现。[此处原文缺失一种细菌名称]仅存在于糖尿病合并高血压队列中。单纯糖尿病队列和糖尿病合并高脂血症队列中最丰富的细菌是[此处原文缺失一种细菌名称]，而糖尿病合并高血压队列和糖尿病合并高血压及高脂血症队列中最丰富的细菌是[此处原文缺失一种细菌名称]。此外，糖尿病合并高血压队列中[此处原文缺失一种细菌名称]的相对丰度显著低于单纯糖尿病队列和糖尿病合并高脂血症队列。几种细菌与参与者的空腹血糖、血压和血脂相关。总之，高血压和/或高脂血症以及其他患者因素可影响糖尿病患者的尿液微生物群组成。本研究的见解可用于开发针对糖尿病患者合并症（包括尿路感染）的基于微生物群的治疗方法。
