Ashraf Aisha, Sarfraz Raja Adil, Rashid Muhammad Abid, Shahid Muhammad
Department of Chemistry, University of Agriculture, Faisalabad 38040, Pakistan.
Department of Chemistry, University of Agriculture, Faisalabad 38040, Pakistan; Central Hi-Tech Laboratory, University of Agriculture, Faisalabad 38040, Pakistan.
J Food Drug Anal. 2015 Mar;23(1):109-115. doi: 10.1016/j.jfda.2014.05.007. Epub 2014 Sep 26.
The aim of present study was to evaluate antioxidant, antimicrobial, and antitumor activities of methanol, hexane, and aqueous extracts of fresh Euphorbia royleana. Total phenolic and flavonoid contents were estimated as gallic acid and querectin equivalents, respectively. Antioxidant activity was assessed by scavenging of free 2,2'- diphenyl-1-picrylhydrazyl radicals and reduction of ferric ions, and it was observed that inhibition values increase linearly with increase in concentration of extract. The results of ferric reducing antioxidant power assay showed that hexane extract has maximum ferric reducing power (12.70 ± 0.49 mg gallic acid equivalents/g of plant extract). Maximum phenolic (47.47 ± 0.71 μg gallic acid equivalents/mg of plant extract) and flavonoid (63.68 ± 0.43 μg querectin equivalents/mg of plant extract) contents were also found in the hexane extract. Furthermore, we examined antimicrobial activity of the three extracts (methanol, hexane, aqueous) against a panel of microorganisms (Escherichia coli, Bacillus subtillis, Pasteurella multocida, Aspergillus niger, and Fusarium solani) by disc-diffusion assay, and found the hexane extract to be the best antimicrobial agent. Hexane extract was also observed as to be most effective in a potato disc assay. As hexane extract showed potent activity in all the investigated assays, it was targeted for cytotoxic assessment. Maximum cytotoxicity (61.66%) by hexane extract was found at 800 μg/mL. It is concluded that investigated extracts have potential for isolation of antioxidant and antimicrobial compounds for the pharmaceutical industry.
本研究的目的是评估新鲜大戟的甲醇提取物、己烷提取物和水提取物的抗氧化、抗菌和抗肿瘤活性。总酚含量和黄酮含量分别以没食子酸当量和槲皮素当量来估算。通过清除2,2'-二苯基-1-苦基肼自由基和还原铁离子来评估抗氧化活性，结果发现抑制值随提取物浓度的增加呈线性增加。铁还原抗氧化能力测定结果表明，己烷提取物具有最大的铁还原能力（12.70±0.49毫克没食子酸当量/克植物提取物）。己烷提取物中还发现了最高的酚类（47.47±0.71微克没食子酸当量/毫克植物提取物）和黄酮类（63.68±0.43微克槲皮素当量/毫克植物提取物）含量。此外，我们通过纸片扩散法检测了三种提取物（甲醇、己烷、水提取物）对一组微生物（大肠杆菌、枯草芽孢杆菌、多杀巴斯德菌、黑曲霉和茄腐镰刀菌）的抗菌活性，发现己烷提取物是最佳抗菌剂。在土豆片试验中也观察到己烷提取物最有效。由于己烷提取物在所有研究的试验中都显示出强效活性，因此对其进行了细胞毒性评估。己烷提取物在800微克/毫升时具有最大细胞毒性（61.66%）。结论是，所研究的提取物有潜力为制药行业分离出抗氧化和抗菌化合物。
