Fontana Gianluca, Gershlak Joshua, Adamski Michal, Lee Jae-Sung, Matsumoto Shion, Le Hau D, Binder Bernard, Wirth John, Gaudette Glenn, Murphy William L
Department of Orthopedics and Rehabilitation, University of Wisconsin School of Medicine and Public Health, Madison, WI, 53705, USA.
Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA, 01609, USA.
Adv Healthc Mater. 2017 Apr;6(8). doi: 10.1002/adhm.201601225. Epub 2017 Mar 20.
The commercial success of tissue engineering products requires efficacy, cost effectiveness, and the possibility of scaleup. Advances in tissue engineering require increased sophistication in the design of biomaterials, often challenging the current manufacturing techniques. Interestingly, several of the properties that are desirable for biomaterial design are embodied in the structure and function of plants. This study demonstrates that decellularized plant tissues can be used as adaptable scaffolds for culture of human cells. With simple biofunctionalization technique, it is possible to enable adhesion of human cells on a diverse set of plant tissues. The elevated hydrophilicity and excellent water transport abilities of plant tissues allow cell expansion over prolonged periods of culture. Moreover, cells are able to conform to the microstructure of the plant frameworks, resulting in cell alignment and pattern registration. In conclusion, the current study shows that it is feasible to use plant tissues as an alternative feedstock of scaffolds for mammalian cells.
组织工程产品的商业成功需要有效性、成本效益以及扩大规模的可能性。组织工程的进展要求生物材料设计更加复杂精密，这常常对当前的制造技术构成挑战。有趣的是，生物材料设计所需的一些特性体现在植物的结构和功能中。本研究表明，脱细胞植物组织可用作培养人类细胞的适应性支架。通过简单的生物功能化技术，能够使人类细胞在多种植物组织上黏附。植物组织的高亲水性和出色的水运输能力使细胞在长时间培养过程中得以扩增。此外，细胞能够顺应植物框架的微观结构，从而实现细胞排列和图案配准。总之，当前研究表明，使用植物组织作为哺乳动物细胞支架的替代原料是可行的。
