Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
Department of Pathology, Radboud Institute for Molecular Life Sciences, Radboud university medical center, Nijmegen, The Netherlands.
AAPS J. 2020 Dec 28;23(1):11. doi: 10.1208/s12248-020-00550-0.
Tissue decellularization yields complex scaffolds with retained composition and structure, and plants offer an inexhaustible natural source of numerous shapes. Plant tissue could be a solution for regenerative organ replacement strategies and advanced in vitro modeling, as biofunctionalization of decellularized tissue allows adhesion of various kinds of human cells that can grow into functional tissue. Here, we investigated the potential of spinach leaf vasculature and chive stems for kidney tubule engineering to apply in tubular transport studies. We successfully decellularized both plant tissues and confirmed general scaffold suitability for topical recellularization with renal cells. However, due to anatomical restrictions, we believe that spinach and chive vasculature themselves cannot be recellularized by current methods. Moreover, gradual tissue disintegration and deficient diffusion capacity make decellularized plant scaffolds unsuitable for kidney tubule engineering, which relies on transepithelial solute exchange between two compartments. We conclude that plant-derived structures and biomaterials need to be carefully considered and possibly integrated with other tissue engineering technologies for enhanced capabilities.
组织脱细胞处理可产生具有保留成分和结构的复杂支架，而植物则提供了无数形状的取之不尽的天然来源。植物组织可能是再生器官替代策略和先进的体外建模的解决方案，因为脱细胞组织的生物功能化允许各种人类细胞的黏附，这些细胞可以生长成功能性组织。在这里，我们研究了菠菜叶脉管系统和韭菜茎用于肾小管工程的潜力，以应用于管状转运研究。我们成功地对这两种植物组织进行了脱细胞处理，并证实了一般支架适合用肾细胞进行局部再细胞化。然而，由于解剖限制，我们认为菠菜和韭菜的脉管系统本身不能通过当前的方法再细胞化。此外，逐渐的组织解体和不足的扩散能力使脱细胞植物支架不适合用于肾小管工程，因为肾小管工程依赖于两个隔室之间的跨上皮溶质交换。我们得出结论，植物衍生的结构和生物材料需要仔细考虑，并可能与其他组织工程技术相结合，以提高其能力。
