Wang Lei, Zhang Yue
Department of Food Science and Technology, University of Nebraska-Lincoln , 1901 North 21st Street, Lincoln, Nebraska 68588, United States.
J Agric Food Chem. 2017 Apr 12;65(14):2990-2998. doi: 10.1021/acs.jafc.7b00194. Epub 2017 Mar 31.
Eugenol-loaded nanoemulsion by zein and sodium caseinate (NaCas) was prepared without using specific equipment or organic solvents. The deprotonated eugenol in hot alkaline was added to NaCas/zein mixtures with different mass ratios at pH 11.5 and then neutralized to pH 7.0. The nanoemulsions showed a well-defined diameter (around 109-139 nm) and a negative surface potential (from -28.5 to -35.8 mV) with spherical morphology. The entrapment efficiency (EE) of 1% (v/v) eugenol reached 84.24% by 2% (m/v) NaCas/zein at a mass ratio of 1:1. This formulation also showed the narrowest size distribution and extraordinary stability during ambient storage (22 °C) up to 30 days and retained good redispersibility after spray- or freeze-drying. The current study showed a promising clean and low-cost strategy to deliver lipophilic compounds containing the hydroxyl group.
采用玉米醇溶蛋白和酪蛋白酸钠（NaCas）制备了负载丁香酚的纳米乳液，无需使用特定设备或有机溶剂。将热碱性条件下的去质子化丁香酚在pH 11.5时加入到不同质量比的NaCas/玉米醇溶蛋白混合物中，然后中和至pH 7.0。纳米乳液呈现出明确的直径（约109 - 139 nm）和负表面电位（-28.5至-35.8 mV），形态为球形。在质量比为1:1的2%（m/v）NaCas/玉米醇溶蛋白体系中，1%（v/v）丁香酚的包封率（EE）达到84.24%。该制剂在环境温度（22°C）下储存30天期间还表现出最窄的粒径分布和非凡的稳定性，并且在喷雾或冷冻干燥后保持良好的再分散性。当前研究展示了一种有前景的清洁且低成本的策略，用于递送含羟基的亲脂性化合物。
