Datta Siraj, Jana Debanjan, Maity Tilak Raj, Samanta Aveek, Banerjee Rajarshi
Department of Biotechnology, Haldia Institute of Technology, Haldia, West Bengal, 721657, India.
3 Biotech. 2016 Jun;6(1):18. doi: 10.1007/s13205-015-0348-8. Epub 2016 Jan 9.
Quorum sensing (QS) plays an important role in virulence of Pseudomonas aeruginosa, blocking of QS ability are viewed as viable antimicrobial chemotherapy and which may prove to be a safe anti-virulent drug. Bioactive components from Piper betle have been reported to possess antimicrobial ability. This study envisages on the anti-QS properties of ethanolic extract of P. betle leaf (PbLE) using P. aeruginosa PAO1 as a model organism. A marked reduction in swarming, swimming, and twitching ability of the bacteria is demonstrated in presence of PbLE. The biofilm and pyocyanin production also shows a marked reduction in presence of PbLE, though it does not affect the bacterial growth. Thus, the studies hint on the possible effect of the bioactive components of PbLE on reducing the virulent ability of the bacteria; identification of bioactive compounds should be investigated further.
群体感应（QS）在铜绿假单胞菌的毒力中起重要作用，阻断QS能力被视为可行的抗菌化疗方法，并且这可能被证明是一种安全的抗毒药物。据报道，蒌叶中的生物活性成分具有抗菌能力。本研究以铜绿假单胞菌PAO1为模式生物，设想研究蒌叶乙醇提取物（PbLE）的抗群体感应特性。在PbLE存在的情况下，细菌的群集、游动和颤动能力显著降低。虽然PbLE不影响细菌生长，但在其存在时生物膜和绿脓菌素的产生也显著减少。因此，这些研究暗示了PbLE的生物活性成分可能对降低细菌的毒力有作用；生物活性化合物的鉴定应进一步研究。
