Vázquez-Martínez Juan, Buitemea-Cantúa Génesis V, Gutierrez-Villagomez Juan Manuel, García-González Julia P, Ramírez-Chávez Enrique, Molina-Torres Jorge
Departamento de Ingeniería Bioquímica, Instituto Tecnológico Superior de Irapuato (ITESI), Tecnológico Nacional de México (TecNM), Irapuato, Guanajuato, Mexico.
Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN) Unidad Irapuato, Irapuato, Guanajuato, Mexico.
Heliyon. 2020 Jan 2;6(1):e03137. doi: 10.1016/j.heliyon.2019.e03137. eCollection 2020 Jan.
In the search of new and safe antibacterial compounds, the quorum sensing system (QS) modulation by natural products has been studied. As a result, many plant-derived compounds have been identified as potent quorum sensing inhibitors. L. (black pepper) ethanolic extract inhibits the QS in some Gram-negative bacteria but the active components have not been previously identified. Thus, the objective of this work was to identify the peppercorns main components that block the QS, applying bioassay and chromatographic techniques. Piperine and trichostachine were identified as the main components responsible for the quorum quenching (QQ) activity of peppercorns extract. Piperine at 30 mg/L, decreased the violacein production by CV026 by 35%, without affecting bacterial growth. Piperine concentration of 40 mg/L decreases violacein production by CV026 by 70% and growth in only 4.34%. Trichostachine at 50 mg/L decreases violacein production by CV026 by 12%, without affecting bacterial growth. xtract concentration of 0.5 g/L decreased violacein production in 40 % and no effects on growth were observed. Neither extract, piperine, nor trichostachine did affect QS of PAO1. Data here described exhibit the potential of piperamides as modulators of QS, not previously reported.
在寻找新型安全抗菌化合物的过程中，人们对天然产物对群体感应系统（QS）的调节作用进行了研究。结果，许多植物来源的化合物已被鉴定为有效的群体感应抑制剂。黑胡椒乙醇提取物可抑制某些革兰氏阴性菌中的群体感应，但此前尚未鉴定出其活性成分。因此，本研究的目的是应用生物测定和色谱技术，确定黑胡椒中阻断群体感应的主要成分。胡椒碱和三氯沙林被鉴定为黑胡椒提取物群体猝灭（QQ）活性的主要成分。30mg/L的胡椒碱可使CV026产生的紫菌素减少35%，而不影响细菌生长。40mg/L的胡椒碱可使CV026产生的紫菌素减少70%，且细菌生长仅减少4.34%。50mg/L的三氯沙林可使CV026产生的紫菌素减少12%，而不影响细菌生长。0.5g/L的提取物浓度可使紫菌素产生减少40%，且未观察到对生长的影响。提取物、胡椒碱和三氯沙林均未影响PAO1的群体感应。此处描述的数据显示了胡椒酰胺作为群体感应调节剂的潜力，这在之前尚未有报道。
