Kumar Ajay, Singh Ritu, Yadav Akhilesh, Giri D D, Singh P K, Pandey Kapil D
Centre of Advance Study in Botany, Banaras Hindu University, Varanasi, 221005, India.
3 Biotech. 2016 Jun;6(1):60. doi: 10.1007/s13205-016-0393-y. Epub 2016 Feb 13.
Fourteen endophytic bacterial isolates were isolated from the rhizome of Curcuma longa L. were characterized on the basis of morphology, biochemical characteristics and 16S rRNA gene sequence analysis. The isolates were identified to six strains namely Bacillus cereus (ECL1), Bacillus thuringiensis (ECL2), Bacillus sp. (ECL3), Bacillus pumilis (ECL4), Pseudomonas putida (ECL5), and Clavibacter michiganensis (ECL6). All the strains produced IAA and solubilized phosphate and only two strains produced siderophore (ECL3 and ECL5) during plant growth promoting trait analysis. All the endophytic strains utilized glucose, sucrose and yeast extract as a carbon source where as glycine, alanine, cystine and glutamine as nitrogen source. The strains were mostly sensitive to antibiotic chloramphenicol followed by erythromycin while resistant to polymixin B. The endophytic strains effectively inhibit the growth of Escherichia coli, Klebsiella pneumoniae and some of the fungal strain like Fusarium solani and Alterneria alternata. The strain ECL2 and ECL4 tolerated maximum 8 % of NaCl concentration where as strains ECL5 and ECL6 6 % in salinity tolerance.
从姜黄根茎中分离出14株内生细菌菌株，并根据形态学、生化特性和16S rRNA基因序列分析对其进行了表征。这些分离菌株被鉴定为6个菌株，即蜡样芽孢杆菌（ECL1）、苏云金芽孢杆菌（ECL2）、芽孢杆菌属（ECL3）、短小芽孢杆菌（ECL4）、恶臭假单胞菌（ECL5）和密执安棒形杆菌（ECL6）。在植物生长促进特性分析中，所有菌株都产生吲哚乙酸并溶解磷，只有两个菌株（ECL3和ECL5）产生铁载体。所有内生菌株都利用葡萄糖、蔗糖和酵母提取物作为碳源，而利用甘氨酸、丙氨酸、胱氨酸和谷氨酰胺作为氮源。这些菌株大多对氯霉素敏感，其次是红霉素，而对多粘菌素B有抗性。内生菌株能有效抑制大肠杆菌、肺炎克雷伯菌以及一些真菌菌株如茄腐镰刀菌和链格孢的生长。菌株ECL2和ECL4能耐受最高8%的氯化钠浓度，而菌株ECL5和ECL6在耐盐性方面为6%。
