Peele K Abraham, Ch V Ravi Teja, Kodali Vidya P
Department of Biotechnology, Vignan's University, Vadlamudi, Guntur Dist., Andhra Pradesh, India.
Department of Biotechnology, Vikrama Simhapuri University, Nellore Dist., Andhra Pradesh, India.
3 Biotech. 2016 Dec;6(2):177. doi: 10.1007/s13205-016-0494-7. Epub 2016 Aug 22.
Biosurfactants produced by biofilm-forming bacteria have great applications in biotechnology, pharmaceutical, food engineering, bioremediation, and biohydrometallurgy industries. This study aimed to find out the bacteria that produce novel exopolymers (EPSs) which can find potential role in oil biodegradation. A screening procedure was performed to detect EPS-producing bacteria. The EPS producing isolate was identified as Acinetobacter species by 16S rDNA analysis. The polymer produced by the isolate has shown significant emulsification and surfactant activities, and the activities were compared to some of the commercial emulsifiers. The EPS has been partially characterized by FTIR analysis and has been proved to be a glycolipoprotein. This is one of the very few reports on Acinetobacter species producing EPS with surfactant properties.
由形成生物膜的细菌产生的生物表面活性剂在生物技术、制药、食品工程、生物修复和生物湿法冶金等行业有广泛应用。本研究旨在找出能产生新型胞外聚合物（EPSs）的细菌，这些聚合物在石油生物降解中可能发挥潜在作用。进行了筛选程序以检测产生EPS的细菌。通过16S rDNA分析将产生EPS的分离株鉴定为不动杆菌属。该分离株产生的聚合物表现出显著的乳化和表面活性剂活性，并将这些活性与一些商业乳化剂进行了比较。通过傅里叶变换红外光谱（FTIR）分析对EPS进行了部分表征，结果证明它是一种糖脂蛋白。这是关于不动杆菌属产生具有表面活性剂特性的EPS的极少数报道之一。
