一种不依赖PCR的转化相关重组方法揭示了海洋链霉菌中杀宇宙菌素生物合成基因簇。
PCR-Independent Method of Transformation-Associated Recombination Reveals the Cosmomycin Biosynthetic Gene Cluster in an Ocean Streptomycete.
作者信息
Larson Charles B, Crüsemann Max, Moore Bradley S
机构信息
Center for Marine Biotechnology & Biomedicine, Scripps Institution of Oceanography, University of California at San Diego , La Jolla, California 92037, United States.
Biomedical Sciences Graduate Program, University of California at San Diego , La Jolla, California 92093, United States.
出版信息
J Nat Prod. 2017 Apr 28;80(4):1200-1204. doi: 10.1021/acs.jnatprod.6b01121. Epub 2017 Mar 23.
Abstract
The transformation-associated recombination cloning methodology facilitates the genomic capture and heterologous expression of natural product biosynthetic gene clusters (BGCs). We have streamlined this procedure by introduction of synthetic DNA gene blocks for the efficient capture of BGCs. We show the successful capture and expression of the aromatic polyketide antitumor agent cosmomycin from streptomycete bacteria and the discovery of new cosmomycin analogues by mass spectral molecular networking.
摘要
转化相关重组克隆方法有助于天然产物生物合成基因簇（BGCs）的基因组捕获和异源表达。我们通过引入合成DNA基因块来高效捕获BGCs，从而简化了这一过程。我们展示了从链霉菌中成功捕获和表达芳香聚酮类抗肿瘤药物科斯莫霉素，并通过质谱分子网络发现了新的科斯莫霉素类似物。
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