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本文引用的文献

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R2oDNA designer: computational design of biologically neutral synthetic DNA sequences.R2oDNA设计器：生物中性合成DNA序列的计算设计
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2
Rapid and reliable DNA assembly via ligase cycling reaction.通过连接酶循环反应实现快速可靠的DNA组装。
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Large-scale de novo DNA synthesis: technologies and applications.大规模从头 DNA 合成：技术与应用。
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Total synthesis of a functional designer eukaryotic chromosome.功能设计真核染色体的全合成。
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Activation and characterization of a cryptic polycyclic tetramate macrolactam biosynthetic gene cluster.激活并表征一个隐匿的多环四肽大环内酯生物合成基因簇。
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FairyTALE: a high-throughput TAL effector synthesis platform.童话：一个高通量转录激活样效应因子合成平台。
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Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination.利用丝氨酸整合酶位点特异性重组快速构建和修饰代谢途径。
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8
High-throughput, cost-effective verification of structural DNA assembly.高通量、经济高效的结构 DNA 组装验证。
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9
One-pot DNA construction for synthetic biology: the Modular Overlap-Directed Assembly with Linkers (MODAL) strategy.一锅法 DNA 构建用于合成生物学：带接头的模块化重叠定向组装（MODAL）策略。
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DNA assembly techniques for next-generation combinatorial biosynthesis of natural products.用于下一代组合生物合成天然产物的 DNA 组装技术。
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DNA组装技术的最新进展。

Recent advances in DNA assembly technologies.

作者信息

Chao Ran, Yuan Yongbo, Zhao Huimin

机构信息

Department of Chemical and Biomolecular Engineering, Institute for Genomic Biology, University of Illinois at Urbana-Champaig, Urbana, IL, USA.

Department of Chemical and Biomolecular Engineering, Institute for Genomic Biology, University of Illinois at Urbana-Champaig, Urbana, IL, USA

出版信息

FEMS Yeast Res. 2015 Feb;15(1):1-9. doi: 10.1111/1567-1364.12171. Epub 2015 Jan 14.

DOI:10.1111/1567-1364.12171
PMID:24903193
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4257898/
Abstract

DNA assembly is one of the most important foundational technologies for synthetic biology and metabolic engineering. Since the development of the restriction digestion and ligation method in the early 1970s, a significant amount of effort has been devoted to developing better DNA assembly methods with higher efficiency, fidelity, and modularity, as well as simpler and faster protocols. This review will not only summarize the key DNA assembly methods and their recent applications, but also highlight the innovations in assembly schemes and the challenges in automating the DNA assembly methods.

摘要

DNA组装是合成生物学和代谢工程中最重要的基础技术之一。自20世纪70年代初限制性消化和连接方法发展以来，人们投入了大量精力来开发效率更高、保真度更高、模块化更强以及协议更简单、更快的DNA组装方法。本综述不仅将总结关键的DNA组装方法及其近期应用，还将突出组装方案的创新以及DNA组装方法自动化面临的挑战。