Ducournau Céline, Nguyen Thi Tl, Carpentier Rodolphe, Lantier Isabelle, Germon Stéphanie, Précausta Flavien, Pisella Pierre-Jean, Leroux Hervé, Van Langendonck Nathalie, Betbeder Didier, Dimier-Poisson Isabelle
Immunologie Parasitaire et Vaccinologie, Biothérapies Anti-Infectieuses, Université de Tours-INRA, UMR1282 Infectiologie et Santé Publique, UFR Pharmacie, F-37000 Tours, France.
Centre International de Recherche sur l'Inflammation de Lille LIRIC -UMR 995 Inserm/Université Lille 2/CHRU Lille. Innovation thérapeutique ciblant l'inflammation. Groupe Nanomédecine, Faculté de Médecine, F-59045 Lille Cedex, France.
Future Microbiol. 2017 Apr;12:393-405. doi: 10.2217/fmb-2016-0146. Epub 2017 Mar 24.
Development of protein vaccine to prevent congenital infection is a major public health priority. Our goal is the design of mucosal synthetic pathogen inducing protective immune responses against congenital toxoplasmosis.
MATERIALS & METHODS: Mice were immunized intranasally, establishing pregnancy and challenging orally. Placental immune response, congenital infection, pup growth, parasitic load rates were studied.
Pups born to vaccinated infected dams had significantly fewer brain cysts, no intraocular inflammation and normal growth. Protection was associated with a placental cellular Th1 response downregulated by IL-6 and correlated with persistence of vaccine for few hours in the nose before being totally eliminated.
Our vaccine conferred high protection against congenital toxoplasmosis. These results provide support for future studies of other congenital vaccine.
开发预防先天性感染的蛋白质疫苗是一项重大的公共卫生优先事项。我们的目标是设计一种黏膜合成病原体，以诱导针对先天性弓形虫病的保护性免疫反应。
对小鼠进行鼻内免疫，使其怀孕并经口进行攻毒。研究胎盘免疫反应、先天性感染、幼崽生长情况以及寄生虫负荷率。
接种疫苗后感染的母鼠所生幼崽的脑囊肿明显减少，无眼内炎症且生长正常。这种保护作用与胎盘细胞Th1反应有关，该反应受到白细胞介素-6的下调，并且与疫苗在鼻腔中在被完全清除之前持续数小时有关。
我们的疫苗对先天性弓形虫病具有高度保护作用。这些结果为未来其他先天性疫苗的研究提供了支持。
