Breathnach R, Benoist C, O'Hare K, Gannon F, Chambon P
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4853-7. doi: 10.1073/pnas.75.10.4853.
Selected regions of cloned EcoRI fragments of the chicken ovalbumin gene have been sequenced. The positions where the sequences coding for ovalbumin mRNA (ov-mRNA) are interrupted in the genome have been determined, and a previously unreported interruption in the DNA sequences coding for the 5' nontranslated region of the messenger has been discovered. Because directly repeated sequences are found at exon-intron boundaries, the nucleotide sequence alone cannot define unique excision-ligation points for the processing of a possible ov-mRNA precursor. However, the sequences in these boundary regions share common features; this leads to the proposal that there are, in fact, unique excision-ligation points common to all boundaries.
已对鸡卵清蛋白基因的克隆EcoRI片段的选定区域进行了测序。确定了基因组中编码卵清蛋白mRNA(ov-mRNA)的序列被中断的位置,并且发现了信使RNA 5'非翻译区编码的DNA序列中一个以前未报道的中断。由于在外显子-内含子边界发现了直接重复序列,仅核苷酸序列不能为可能的ov-mRNA前体的加工定义独特的切除-连接点。然而,这些边界区域的序列具有共同特征;这导致提出实际上所有边界都存在独特的切除-连接点。