鸡卵清蛋白裂解基因中编码序列和间隔序列的组织方式。

Organization of coding and intervening sequences in the chicken ovalbumin split gene.

作者信息

Mandel J L, Breathnach R, Gerlinger P, Le Meur M, Gannon F, Chambon P

出版信息

Cell. 1978 Jul;14(3):641-53. doi: 10.1016/0092-8674(78)90248-9.

Abstract

The interruptions in the chicken ovalbumin gene which were reported previously (Breathnach, Mandel and Chambon, 1977) are shown to be due to the presence of intervening sequences which separate the messenger-coding sequences. We present evidence for an additional interruption of the gene, which, together with those reported earlier and by Garapin et al. (1978b), make a total of six intervening sequences. All of these intervening sequences are located in the DNA region that corresponds to the part of the ov mRNA which codes for amino acids. The seven coding fragments of the split ovalbumin gene are arranged in the same order and relative orientation as in the ovalbumin double-stranded cDNA. All the sequences coding for ov mRNA are contained in a chromosomal DNA region of 6000 bp, which is more than 3 times longer than ov mRNA. The general organization of the ovalbumin split gene is discussed.

摘要

先前报道的鸡卵清蛋白基因中的中断情况（Breathnach、Mandel和Chambon，1977年）表明是由于存在间隔序列，这些间隔序列将信使编码序列分隔开。我们提供了该基因存在额外中断的证据，连同先前报道的以及Garapin等人（1978b）报道的中断情况，总共形成了六个间隔序列。所有这些间隔序列都位于与ov mRNA编码氨基酸部分相对应的DNA区域。裂解的卵清蛋白基因的七个编码片段的排列顺序和相对方向与卵清蛋白双链cDNA中的相同。所有编码ov mRNA的序列都包含在一个6000 bp的染色体DNA区域中，该区域比ov mRNA长3倍多。本文讨论了卵清蛋白裂解基因的总体结构。