College of Pharmacy, Jiamusi University, Jiamusi 154007, People's Republic of China.
College of Pharmacy, Jiamusi University, Jiamusi 154007, People's Republic of China.
Talanta. 2017 May 15;167:236-241. doi: 10.1016/j.talanta.2017.02.022. Epub 2017 Feb 9.
The antibiotic residue in animal source foods (milk, meat, etc.) is threatening people's health due to its abusing in livestock breeding more and more seriously. In this study, a simple and sensitive SERS method coupled with a two-step pretreatment process of sample was proposed for the residue detection of penicillin G (PENG) in real milk sample. It can be found that the two-step pretreatment process of sample is an essential procedure for the successful detection of PENG residue in milk, which can effectively avoid interference from other components in the sample and achieve the trace-level detection of PENG residue by SERS. Under the optimal test conditions, the limit of detection of PENG residue is 2.54×10mol/L (equal to 0.85μg/kg), which is lower than the standard of the European Union (4μg/kg). And, there is a good linear relationship (R=0.9902) in the concentration range of 1.0×10~1.0×10mol/L. By this method, the recovery of PENG residue ranges from 76% to 97% with relative standard deviation between 4.8% and 2.1%. The proposed SERS method can be effectively applied for determination of PENG residue in milk.
动物源食品（牛奶、肉类等）中的抗生素残留由于畜牧业中抗生素的滥用越来越严重，正在威胁着人们的健康。本研究提出了一种简单、灵敏的 SERS 方法，并结合样品的两步预处理过程，用于检测牛奶中青霉素 G（PENG）的残留。可以发现，样品的两步预处理过程是成功检测牛奶中 PENG 残留的必要程序，它可以有效地避免样品中其他成分的干扰，并通过 SERS 实现 PENG 残留的痕量检测。在最佳测试条件下，PENG 残留的检测限为 2.54×10mol/L（相当于 0.85μg/kg），低于欧盟标准（4μg/kg）。并且，在 1.0×10~1.0×10mol/L 的浓度范围内有良好的线性关系（R=0.9902）。采用该方法，PENG 残留的回收率在 76%至 97%之间，相对标准偏差在 4.8%至 2.1%之间。所提出的 SERS 方法可有效用于牛奶中 PENG 残留的检测。
