College of Basic Education, Al-Mustansiriyah University, Baghdad, Iraq.
College of Basic Education, Al-Mustansiriyah University, Baghdad, Iraq.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Jan 5;206:31-36. doi: 10.1016/j.saa.2018.07.103. Epub 2018 Aug 1.
A new method for the detection of ultra-low concentration of penicillin G (PG) antibiotic was innovated using gold nanoparticles/porous silicon (AuNPs/PSi) SERS active substrate. PSi was employed as a template and a reducing agent to deposited aggregated AuNPs with a high density of hot spot regions. A highly enhancement performance and reproducible AuNPs/PSi SERS substrate was fabricated. The AuNPs/PSi SERS substrate was investigated for detection PG drug at different concentrations ranging from 10 to 10 M. The results showed that the proposed AuNPs/PSi SERS substrate provide an efficient way for detection the lowest concentration of PG. The adsorption process of the PG drug on the surface of the AuNPs/PSi was investigated, it was found that PG was close to the surface of AuNPs/PSi through the carboxylate group. The enhancement factor (EF) of 5 × 10 and a 4.5% relative standard deviation of reproducibility were obtained at an ultra-low PG concentration of 10 M. The influence of the pH value on the EF for PG antibiotic under acidic and alkaline conditions (EF) was studied, it was shown that the highest EF with efficient activity toward PG was obtained at pH = 5.
一种新的检测超低浓度青霉素 G（PG）抗生素的方法被创新出来，使用了金纳米粒子/多孔硅（AuNPs/PSi）SERS 活性衬底。PSi 被用作模板和还原剂，以沉积高密度热点区域的聚集 AuNPs。制备了具有高增强性能和重现性的 AuNPs/PSi SERS 衬底。研究了 AuNPs/PSi SERS 衬底对不同浓度（10-10 M）PG 药物的检测性能。结果表明，所提出的 AuNPs/PSi SERS 衬底为检测最低浓度的 PG 提供了一种有效的方法。研究了 PG 药物在 AuNPs/PSi 表面的吸附过程，发现 PG 通过羧酸盐基团接近 AuNPs/PSi 表面。在超低 PG 浓度为 10 M 时，获得了 5×10 的增强因子（EF）和 4.5%的重现性相对标准偏差。研究了在酸性和碱性条件下（EF）pH 值对 PG 抗生素 EF 的影响，结果表明，在 pH=5 时获得了对 PG 最有效的最高 EF。
