Keane R W, Mehta P P, Rose B, Honig L S, Loewenstein W R, Rutishauser U
Department of Physiology and Biophysics, University of Miami School of Medicine, Florida 33101.
J Cell Biol. 1988 Apr;106(4):1307-19. doi: 10.1083/jcb.106.4.1307.
We studied the development of NCAM and gap junctional communication, and their mutual relationship in chick neuroectoderm in vitro. Expression of NCAM, as detected by monoclonal and polyclonal antibodies, and development of junctional communication, as detected by extensive cell-to-cell transfer of 400-500-D fluorescent tracers, occurred in cultures from stage-2 embryos onward. Both expressions presumably required primary induction. The differentiating cells formed discrete fields of expression on the second to third day in culture, with the NCAM fields coinciding with the junctional communication fields delineated by the tracers. Other neural differentiations developed in the following order: tetanus toxin receptors, neurofilament protein, and neurite outgrowth. Chronic treatment with antibody Fab fragments against NCAM interfered with the development of communication, suggesting that NCAM-mediated adhesion promotes formation of cell-to-cell channels. Temperature-sensitive mutant Rous sarcoma virus blocked (reversibly) communication and the subsequent development of neurofilament protein and neurites, but expression of NCAM continued.
我们研究了神经细胞黏附分子（NCAM）和缝隙连接通讯在鸡胚神经外胚层体外培养中的发育情况及其相互关系。通过单克隆抗体和多克隆抗体检测到的NCAM表达，以及通过400 - 500道尔顿荧光示踪剂在细胞间的广泛转移检测到的连接通讯的发育，从2期胚胎的培养物开始就出现了。这两种表达可能都需要初级诱导。在培养的第二天到第三天，分化细胞形成了离散的表达区域，NCAM区域与示踪剂描绘的连接通讯区域重合。其他神经分化按以下顺序发展：破伤风毒素受体、神经丝蛋白和神经突生长。用抗NCAM抗体Fab片段进行长期处理会干扰通讯的发育，这表明NCAM介导的黏附促进了细胞间通道的形成。温度敏感型劳氏肉瘤病毒（可逆地）阻断了通讯以及随后神经丝蛋白和神经突的发育，但NCAM的表达仍在继续。
