Zhang Hong-Tao, Tian Miao, He Qiao-Wei, Chi Nan, Xiu Chun-Ming, Wang Yun-Bo
Department of Neurosurgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, China.
Department of Cardiology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, China.
Saudi Pharm J. 2017 Mar;25(3):370-375. doi: 10.1016/j.jsps.2016.09.001. Epub 2016 Sep 14.
Neuroinflammation is the commonest cause of neurodegenerative diseases such as Alzheimer's disease. Present investigation evaluates the inhibitory effect of ethanolic root extract of (AS) on inflammatory mediators production in lipopolysaccharide (LPS) stimulated C6 cells. C6 cells were treated with AS (20 and 40 mg/kg) and nimesulide (NSL, 1.5 μg/ml) for 1 day. Thereafter various parameters such as production of ROS, release of nitrite, MDA, glutathione level and NF-κB translocation were estimated in C6 cell lines. Effect of AS was estimated on the expressions of tumor necrosis factor α (TNF-α) of human monocytic leukemia cell line (THP-1). It was observed that AS (20 and 40 mg/ml) treated group shows significant ( < 0.01) decrease in production of ROS, Nitrite release and MDA level in LPS activated C6 cell lines compared to negative control group. Moreover, treatment with it decreases glutathione level and inhibits the translocation of NF-κB in LPS activated C6 cell lines compared to negative control group. There were significant ( < 0.01) decreases in expression of TNF-α in AS treated group compared to negative control group in THP-1 cell lines. Present investigation concludes the anti neuroinflammatory effect of ethanolic extract of AS root by decreasing oxidative stress and attenuates the cytokine.
神经炎症是阿尔茨海默病等神经退行性疾病最常见的病因。目前的研究评估了[植物名称]（AS）乙醇根提取物对脂多糖（LPS）刺激的C6细胞中炎症介质产生的抑制作用。将C6细胞用AS（20和40mg/kg）和尼美舒利（NSL，1.5μg/ml）处理1天。此后，在C6细胞系中评估了各种参数，如活性氧的产生、亚硝酸盐的释放、丙二醛、谷胱甘肽水平和核因子κB易位。评估了AS对人单核细胞白血病细胞系（THP-1）肿瘤坏死因子α（TNF-α）表达的影响。观察到，与阴性对照组相比，AS（20和40mg/ml）处理组在LPS激活的C6细胞系中活性氧的产生、亚硝酸盐释放和丙二醛水平显著降低（P<0.01）。此外，与阴性对照组相比，用其处理可降低LPS激活的C6细胞系中的谷胱甘肽水平并抑制核因子κB的易位。在THP-1细胞系中，与阴性对照组相比，AS处理组中TNF-α的表达显著降低（P<0.01）。目前的研究得出结论，AS根乙醇提取物通过降低氧化应激和减轻细胞因子发挥抗神经炎症作用。
