Smutny Michael, Ákos Zsuzsa, Grigolon Silvia, Shamipour Shayan, Ruprecht Verena, Čapek Daniel, Behrndt Martin, Papusheva Ekaterina, Tada Masazumi, Hof Björn, Vicsek Tamás, Salbreux Guillaume, Heisenberg Carl-Philipp
Institute of Science and Technology Austria, Am Campus 1, A-3400 Klosterneuburg, Austria.
Department of Biological Physics, Eötvös University, Pázmány Péter sétány 1A, Budapest H-1117, Hungary.
Nat Cell Biol. 2017 Apr;19(4):306-317. doi: 10.1038/ncb3492. Epub 2017 Mar 27.
During embryonic development, mechanical forces are essential for cellular rearrangements driving tissue morphogenesis. Here, we show that in the early zebrafish embryo, friction forces are generated at the interface between anterior axial mesoderm (prechordal plate, ppl) progenitors migrating towards the animal pole and neurectoderm progenitors moving in the opposite direction towards the vegetal pole of the embryo. These friction forces lead to global rearrangement of cells within the neurectoderm and determine the position of the neural anlage. Using a combination of experiments and simulations, we show that this process depends on hydrodynamic coupling between neurectoderm and ppl as a result of E-cadherin-mediated adhesion between those tissues. Our data thus establish the emergence of friction forces at the interface between moving tissues as a critical force-generating process shaping the embryo.
在胚胎发育过程中，机械力对于驱动组织形态发生的细胞重排至关重要。在此，我们表明，在斑马鱼早期胚胎中，在前轴中胚层（前索板，ppl）祖细胞朝着动物极迁移与神经外胚层祖细胞朝着胚胎植物极相反方向移动的界面处会产生摩擦力。这些摩擦力导致神经外胚层内细胞的整体重排，并决定神经原基的位置。通过实验和模拟相结合的方法，我们表明这一过程依赖于神经外胚层和ppl之间的流体动力耦合，这是由于这些组织之间E-钙黏蛋白介导的黏附作用。因此，我们的数据证实了运动组织界面处摩擦力的出现是塑造胚胎的关键力产生过程。
