Nieto Daniel, McGlynn Peter, de la Fuente María, Lopez-Lopez Rafael, O'connor Gerard M
Microoptics and GRIN Optics Group, Applied Physics Department, Faculty of Physics, University of Santiago de Compostela, Santiago de Compostela, E15782 Spain; School of Physics, National Centre for Laser Applications, National University of Ireland, University Road, Galway, Ireland.
School of Physics, National Centre for Laser Applications, National University of Ireland, University Road, Galway, Ireland.
Colloids Surf B Biointerfaces. 2017 Jun 1;154:263-269. doi: 10.1016/j.colsurfb.2017.03.043. Epub 2017 Mar 22.
Microfluidic chips have demonstrated their significant application potentials in microbiological processing and chemical reactions, with the goal of developing monolithic and compact chip-sized multifunctional systems. Heat generation and thermal control are critical in some of the biochemical processes. The paper presents a laser direct-write technique for rapid prototyping and manufacturing of microheater chips and its applicability for lab-on-a-chip cell culture outside a cell incubator. The aim of the microheater is to take the role of conventional incubators for cell culture for facilitating microscopic observation and/or other online monitoring activities during cell culture and provides portability of cell culture operation. Microheaters (5mm×5mm) have been successfully fabricated on soda-lime glass substrates covered with aluminium layer of thickness 120nm. Experimental results show that the microheaters exhibit good performance in temperature rise and decay characteristics, with localized heating at targeted spatial domains. These microheaters were suitable for a maximum long-term operation temperature of 120°C and validated for operation at 37°C for 48h. Results demonstrated that the microheaters are suitable for the culture of immortalised cell lines. The growth and viability of SW480 colon adenocarcinoma cells cultured the developed microheater chip were comparable to the results obtained in a conventional cell incubator.
微流控芯片已在微生物处理和化学反应中展现出巨大的应用潜力，目标是开发单片式且紧凑的芯片尺寸多功能系统。在一些生化过程中，发热和热控制至关重要。本文介绍了一种用于微加热器芯片快速原型制作和制造的激光直写技术及其在细胞培养箱外的芯片实验室细胞培养中的适用性。微加热器的目的是替代传统的细胞培养箱，以便在细胞培养过程中便于显微镜观察和/或其他在线监测活动，并提供细胞培养操作的便携性。已在覆盖有厚度为120nm铝层的钠钙玻璃基板上成功制造出尺寸为5mm×5mm的微加热器。实验结果表明，这些微加热器在升温及降温特性方面表现良好，能在目标空间域实现局部加热。这些微加热器的最高长期工作温度为120°C，并已验证在37°C下可运行48小时。结果表明，这些微加热器适用于永生化细胞系的培养。在已开发的微加热器芯片上培养的SW480结肠腺癌细胞的生长和活力与在传统细胞培养箱中获得的结果相当。
