BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland.
In Vitro Toxicology Group, Swansea University Medical School, Wales, UK.
Sci Rep. 2017 Mar 27;7(1):434. doi: 10.1038/s41598-017-00369-4.
Determination of the cell type specific response is essential towards understanding the cellular mechanisms associated with disease states as well as assessing cell-based targeting of effective therapeutic agents. Recently, there have been increased calls for advanced in vitro multi-cellular models that provide reliable and valuable tools correlative to in vivo. In this pursuit the ability to assess the cell type specific response is imperative. Herein, we report a novel approach towards resolving each specific cell type of a multi-cellular model representing the human lung epithelial tissue barrier via multi-colour flow cytometry (FACS). We proved via ≤ five-colour FACS that the manipulation of this in vitro model allowed each cell type to be resolved with no impact upon cell viability. Subsequently, four-colour FACS verified the ability to determine the biochemical effect (e.g. oxidative stress) of each specific cell type. This technique will be vital in gaining information upon cellular mechanics when using next-level, multi-cellular in vitro strategies.
确定细胞类型特异性反应对于理解与疾病状态相关的细胞机制以及评估基于细胞的有效治疗药物靶向至关重要。最近，人们越来越呼吁开发先进的体外多细胞模型，这些模型提供与体内相关的可靠和有价值的工具。在这方面，评估细胞类型特异性反应的能力至关重要。在这里，我们报告了一种通过多色流式细胞术（FACS）解析代表人类肺上皮组织屏障的多细胞模型中每种特定细胞类型的新方法。我们通过≤五色 FACS 证明，对这种体外模型的操作允许解析每种细胞类型，而不会对细胞活力产生影响。随后，四色 FACS 验证了确定每种特定细胞类型的生化效应（例如氧化应激）的能力。当使用下一代多细胞体外策略时，这项技术对于获取有关细胞力学的信息将是至关重要的。
