Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700, Fribourg, Switzerland.
Department of Chemistry, University of Fribourg, Chemin du Musée 9, 1700, Fribourg, Switzerland.
Sci Rep. 2018 Jun 29;8(1):9861. doi: 10.1038/s41598-018-28206-2.
Three dimensional (3D) co-cultures to mimic cellular dynamics have brought significant impacts in tissue engineering approaches for biomedical research. Herein, we present a novel sample holder combined with time-lapse fluorescence imaging technique, referred as 4D live cell imaging, allowing direct visualization of various cells up to 24 hours. We further extended our approach to monitor kinetics and dynamics of particle uptake by cells and translocation across tissue membranes.
三维（3D）共培养物模拟细胞动力学在组织工程方法的生物医学研究中带来了重大影响。在此，我们提出了一种新颖的样品架，结合延时荧光成像技术，即 4D 活细胞成像，可直接观察长达 24 小时的各种细胞。我们进一步扩展了我们的方法，以监测细胞摄取颗粒的动力学和动态以及跨组织膜的转运。
