Rijal Girdhari, Shin Hong-In
Department of Oral Pathology & Regenerative Medicine, School of Dentistry, Kyungpook National University, Taegu 704412, South Korea.
Department of Biomedical Science, Elson S. Floyd College of Medicine, Washington State University, Spokane, WA 99210, USA.
Regen Med. 2017 Apr;12(3):263-273. doi: 10.2217/rme-2016-0147. Epub 2017 Mar 28.
The present study was conducted to evaluate the efficacy of human dentine grafts for new bone augmentation.
MATERIALS & METHODS: Dentine grafts (demineralized dentine matrix [DDM] and mineralized dentine matrix [MDM]) were prepared and implanted in rats. Tetracycline was administered twice. Paraffin and resin sections were prepared from the harvested grafts and stained respectively with hematoxylin and eosin (in addition to tartrate acid phosphatase for osteoclasts) and Villanueva. The new bone formation (bone thickness, mineral apposition rate and the bone formation rate) was analyzed in tetracycline-labeled resin sections.
RESULTS & CONCLUSION: DDM grafts implanted in bone were better able to augment the bone compared to MDM grafts. However, both MDM and DDM failed to induce new bone in ectopic site, they could be considered as alternative autograft substitutes after protocol optimization.
本研究旨在评估人牙本质移植物用于新骨增量的疗效。
制备牙本质移植物（脱矿牙本质基质[DDM]和矿化牙本质基质[MDM]）并植入大鼠体内。给予四环素两次。从收获的移植物制备石蜡切片和树脂切片，分别用苏木精和伊红染色（除了用于破骨细胞的酒石酸磷酸酶）以及Villanueva染色。在四环素标记的树脂切片中分析新骨形成情况（骨厚度、矿物质沉积率和骨形成率）。
与MDM移植物相比，植入骨内的DDM移植物在增加骨量方面表现更佳。然而，MDM和DDM在异位部位均未能诱导新骨形成，经过方案优化后它们可被视为自体移植替代物的备选材料。
