Dutta S K, Verma M, Verma M
Department of Botany, Genetics and Human Genetics, Howard University, Washington, DC 20059.
Curr Genet. 1987;12(3):225-9. doi: 10.1007/BF00436884.
Neurospora crassa wild type genome shows DNA sequences which are homologous to the sequences present in the rRNA processing genes of the yeast Saccharomyces cerevisiae. Five such processing genes from yeast, viz., RNA1 through RNA5, cloned in plasmid pBR322 were transformed in Escherichia coli strain LE392. Southern blots containing DNAs from these clones were restricted with several restriction endonucleases along with DNAs from lambda phage, rice (plant) and neuroblastoma (animal), were hybridized with 32P-labelled nick-translated N. crassa nuclear DNA under very stringent conditions. Autoradiograms of these blots revealed that four yeast rRNA processing genes (RNA1, RNA2, RNA3, and RNA4) showed homology with N. crassa nuclear DNA but such analogs were not found in DNAs representing prokaryotes, phages, higher plants and animals.
粗糙脉孢菌野生型基因组显示出与酿酒酵母rRNA加工基因中存在的序列同源的DNA序列。酵母的五个此类加工基因，即RNA1至RNA5，克隆于质粒pBR322中，并转化到大肠杆菌LE392菌株中。含有这些克隆DNA的Southern印迹用几种限制性内切酶进行酶切，同时用来自λ噬菌体、水稻（植物）和神经母细胞瘤（动物）的DNA，在非常严格的条件下与32P标记的经切口平移的粗糙脉孢菌核DNA杂交。这些印迹的放射自显影片显示，四个酵母rRNA加工基因（RNA1、RNA2、RNA3和RNA4）与粗糙脉孢菌核DNA显示出同源性，但在代表原核生物、噬菌体、高等植物和动物的DNA中未发现此类类似物。
