Chambers C, Dutta S K, Crouch R J
Gene. 1986;44(1):159-64. doi: 10.1016/0378-1119(86)90057-0.
Using [32P]DNA probes from a clone containing 17S, 5.8S and 26S rRNA of Neurospora crassa, the remainder of the repeat unit (RU) for ribosomal DNA (rDNA) has been cloned. Combining restriction analysis of the cloned DNA and restriction digests of genomic DNA, the RU was found to be 8.7 kb. The nucleotide sequence was determined for the internal transcribed spacer (ITS) regions one and two, for 5.8S rRNA and for portions of 17S and 26S rRNAs immediately flanking the ITS regions, and compared to the corresponding region of Saccharomyces carlsbergensis. In addition, a comparative restriction analysis of two other Neurospora species was performed using twelve restriction endonucleases. Genomic DNA blots of rDNA from N. intermedia and N. sitophila revealed rDNA RUs of 8.4 kb. The majority of differences in restriction patterns were confined to sequences outside the mature rRNA regions. However, one SmaI recognition site was found in 26S rRNA of N. crassa and N. sitophila but not in N. intermedia.
利用来自包含粗糙脉孢菌17S、5.8S和26S rRNA的克隆的[32P]DNA探针，已克隆出核糖体DNA（rDNA）重复单元（RU）的其余部分。通过对克隆DNA的限制性分析和基因组DNA的限制性酶切，发现RU为8.7 kb。测定了内部转录间隔区（ITS）区域1和区域2、5.8S rRNA以及紧邻ITS区域的17S和26S rRNA部分的核苷酸序列，并与卡尔酵母的相应区域进行了比较。此外，使用12种限制性内切酶对另外两种脉孢菌进行了比较限制性分析。中间脉孢菌和嗜坐脉孢菌rDNA的基因组DNA印迹显示rDNA的RU为8.4 kb。限制性图谱的大多数差异局限于成熟rRNA区域之外的序列。然而，在粗糙脉孢菌和嗜坐脉孢菌的26S rRNA中发现了一个SmaI识别位点，而在中间脉孢菌中未发现。
