Wang Lihua, Fu Shihong, Cao Yuxi, Zhang Hailin, Feng Yun, Yang Weihong, Nie Kai, Ma Xuejun, Liang Guodong
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China.
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China.
Emerg Microbes Infect. 2017 Mar 29;6(3):e14. doi: 10.1038/emi.2016.140.
To investigate bat coronaviruses (CoVs), we collected 132 rectal swabs and urine samples from five bat species in three countries in southwestern China. Seven CoVs belonging to distinct groups of severe acute respiratory syndrome (SARS)-like CoVs and α-CoVs were detected in samples from least horseshoe bats. Samples from other bat species were negative for these viruses, indicating that the least horseshoe bat represents one of the natural reservoirs and mixers for strains of CoVs and has a pivotal role in the evolution and dissemination of these viruses. The genetic and evolutionary characteristics of these strains were described. Whole-genome sequencing of a new isolate (F46) from a rectal swab from a least horseshoe bat showed that it contained 29 699 nucleotides, excluding the poly (A) tail, with 13 open reading frames (ORFs). Phylogenetic and recombination analyses of F46 provided evidence of natural recombination between bat SARS-like CoVs (Rs3367 and LYRa11) or SARS-CoV (BJ01), suggesting that F46 could be a new recombinant virus from SARS-like CoVs or SARS-CoVs.
为了研究蝙蝠冠状病毒（CoVs），我们在中国西南部三个国家的五种蝙蝠中收集了132份直肠拭子和尿液样本。在至少菊头蝠的样本中检测到7种属于严重急性呼吸综合征（SARS）样CoVs和α-CoVs不同组别的冠状病毒。来自其他蝙蝠物种的样本对这些病毒呈阴性，这表明菊头蝠是CoV毒株的天然宿主和混合宿主之一，并且在这些病毒的进化和传播中起关键作用。描述了这些毒株的遗传和进化特征。对一只菊头蝠直肠拭子中的新分离株（F46）进行全基因组测序表明，其不含多聚（A）尾时含有29699个核苷酸，有13个开放阅读框（ORF）。F46的系统发育和重组分析提供了蝙蝠SARS样CoVs（Rs3367和LYRa11）或SARS-CoV（BJ01）之间自然重组的证据，表明F46可能是一种来自SARS样CoVs或SARS-CoVs的新型重组病毒。
