Cvetković Tatjana, Veličković-Radovanović Radmila, Stojanović Dijana, Stefanović Nikola, Ignjatović Aleksandra, Stojanović Ivana, Sladojević Nikola, Pavlović Dušica
Institute of Biochemistry, Medical Faculty, University of Niš, Serbia; Clinic for Nephrology, Dialysis and Transplantation, Clinical Centre Niš, Serbia.
Clinic for Nephrology, Dialysis and Transplantation, Clinical Centre Niš, Serbia; Pharmacy Department, Medical Faculty, University of Niš, Serbia.
J Med Biochem. 2015 Jul;34(3):295-303. doi: 10.2478/jomb-2014-0047. Epub 2015 Jul 14.
The aim of the study was to evaluate parameters of oxidative and nitrosative stress as well as antioxidative parameters in a group of renal transplant recipients with stable graft function and no clinical signs of cardiovascular disease. We also aimed to determine the correlations among these parameters and to evaluate potential differences in all the biomarkers with regard to the immunosuppression protocol.
We enrolled 57 renal transplant recipients and 31 controls who were age and sex matched with the renal transplant recipients. All of the patients included in this study had post-renal transplant surgery at least 12 months earlier and were on standard immunosuppressive therapy. In this study, we determined thiobarbituric acid-reactive substances in plasma and red blood cells and advanced oxidation protein products, nitrosative stress parameters (asymmetric and symmetric dimethylarginine - ADMA and SDMA), and antioxidative parameters (total SH groups and catalase activity).
The results of our study demonstrated that the levels of oxidative and nitrosative stress were significantly increased compared to the healthy population (p<0.01 except for plasma catalase activity p<0.05). Correlation analysis showed significant positive correlations between: ADMA and SDMA (p<0.01); ADMA and nitrates (p<0.05); SDMA and nitrates (p<0.05); between OS parameters in the experimental group; AOPP and SH groups (p<0.05) and TBARS in plasma and SH groups (p<0.01), SDMA and AOPP (p< 0.05); SDMA and TBARS in plasma (p<0.05); SDMA and SH groups (p<0.01); nitrates and SH groups (p<0.05).
There was no significant difference in oxidative and nitrosative stress parameters with respect to the immunosuppressive protocol.
本研究旨在评估一组移植肾功能稳定且无心血管疾病临床症状的肾移植受者的氧化应激和亚硝化应激参数以及抗氧化参数。我们还旨在确定这些参数之间的相关性，并评估所有生物标志物在免疫抑制方案方面的潜在差异。
我们纳入了57名肾移植受者和31名与肾移植受者年龄和性别匹配的对照组。本研究纳入的所有患者均在肾移植手术后至少12个月，且正在接受标准免疫抑制治疗。在本研究中，我们测定了血浆和红细胞中的硫代巴比妥酸反应性物质、晚期氧化蛋白产物、亚硝化应激参数（不对称和对称二甲基精氨酸 - ADMA和SDMA）以及抗氧化参数（总SH基团和过氧化氢酶活性）。
我们的研究结果表明，与健康人群相比，氧化应激和亚硝化应激水平显著升高（除血浆过氧化氢酶活性p<0.05外，p<0.01）。相关性分析显示：ADMA与SDMA之间存在显著正相关（p<0.01）；ADMA与硝酸盐之间存在显著正相关（p<0.05）；SDMA与硝酸盐之间存在显著正相关（p<0.05）；实验组的氧化应激参数之间存在显著正相关；AOPP与SH基团之间存在显著正相关（p<0.05），血浆中的TBARS与SH基团之间存在显著正相关（p<0.01），SDMA与AOPP之间存在显著正相关（p<0.05）；血浆中的SDMA与TBARS之间存在显著正相关（p<0.05）；SDMA与SH基团之间存在显著正相关（p<0.01）；硝酸盐与SH基团之间存在显著正相关（p<0.05）。
氧化应激和亚硝化应激参数在免疫抑制方案方面无显著差异。
