Đuričić Ivana, Kotur-Stevuljević Jelena, Miljković Milica, Kerkez Mirko, Đorđević Vladimir, Đurašić Ljubomir, Šobajić Slađana
Department of Bromatology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
J Med Biochem. 2015 Jul;34(3):304-313. doi: 10.2478/jomb-2014-0039. Epub 2015 Jul 14.
This study investigated the effects of a nutritionally relevant intake of eicosapentaenoic (EPA) and docosahexaenoic (DHA) fatty acids derived from oily fish or a fish oil supplement on selected cardiovascular risk factors in average middle-aged individuals.
Thirty-three participants were randomized to receive salmon (oily fish) providing 274 mg EPA + 671 mg DHA/day or a commercial fish oil supplement providing 396 mg EPA + 250 mg DHA/day in a cross-over trial over an 8-week period separated by a 6-month washout period. Blood samples were collected before and after each intervention and lipids, inflammatory and oxidative stress parameters were determined.
Plasma levels of EPA, DHA and total n-3 fatty acids significantly increased after both interventions. A decreasing trend in triglycerides was more pronounced with salmon than with the fish oil supplement, but the changes noticed were not significant. Although there were no relevant changes in inflammatory marker concentrations at the end of both interventions, significant negative correlations were noticed between total plasma n-3 fatty acids and soluble intercellular adhesion molecule and C-reactive protein throughout the whole intervention period (p<0.05). Among the oxidative stress parameters, intervention with salmon showed a prooxidative effect through a superoxide anion increase (p=0.025). A relevant positive correlation was also found between its concentration and total plasma n-3 fatty acids (p<0.05). Other oxidative stress markers were not significantly influenced by the dietary interventions applied.
Following two sets of recommendations for n-3 fatty acids intake aimed at the general public had only a moderate effect on the selected cardiovascular risk factors in average healthy middle-aged subjects over a short-term period.
本研究调查了从油性鱼类或鱼油补充剂中摄入营养相关量的二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）脂肪酸对普通中年个体某些心血管危险因素的影响。
33名参与者被随机分为两组，在为期8周的交叉试验中，一组每天摄入提供274毫克EPA + 671毫克DHA的三文鱼（油性鱼类），另一组每天摄入提供396毫克EPA + 250毫克DHA的市售鱼油补充剂，两组之间有6个月的洗脱期。在每次干预前后采集血样，并测定血脂、炎症和氧化应激参数。
两种干预后，血浆中EPA、DHA和总n-3脂肪酸水平均显著升高。三文鱼组甘油三酯的下降趋势比鱼油补充剂组更明显，但观察到的变化不显著。虽然两种干预结束时炎症标志物浓度没有相关变化，但在整个干预期内，血浆总n-3脂肪酸与可溶性细胞间粘附分子和C反应蛋白之间存在显著的负相关（p<0.05）。在氧化应激参数中，三文鱼干预通过超氧阴离子增加显示出促氧化作用（p=0.025）。其浓度与血浆总n-3脂肪酸之间也发现了显著的正相关（p<0.05）。其他氧化应激标志物未受到所应用饮食干预的显著影响。
针对普通公众的两组n-3脂肪酸摄入建议，在短期内对普通健康中年受试者的某些心血管危险因素仅有适度影响。
