Harahap Urip, Hasibuan Poppy Anjelisa Zaitun, Sitorus Panal, Arfian Nur, Satria Denny
Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Indonesia. Email:
Asian Pac J Cancer Prev. 2018 Feb 26;19(2):565-569. doi: 10.22034/APJCP.2018.19.2.565.
Objective: This study was carried out to investigate the antimigration activity of Zanthoxylum acanthopodium DC. in the 4T1 breast cancer cell line. Methods: Zanthoxylum acanthopodium DC. fruit powder was extracted by maceration method with n-hexane and ethylacetate solvents. Cytotoxicity and proliferation were assessed using the MTT method and the cell cycle by flow cytometry. In addition, wound healing assays were conducted by a microscopic method, and expression of COX-2 and VEGFR-2 were determined using qRT-PCR. Results: The IC50 of the ethylacetate fraction (EAF) was 48.1 ± 1.06 μg/mL. The EAE at a concentration 10 μg/mL with viable cells was 62.3 ± 0.28% after 72 h incubation, with accumulation in the G2-M phase, inhibition of cell migration in the wound healing assay, and decrease in expression of COX-2 (0.62 ± 0.01) and VEGFR-2 (0.39 ± 0.003). Conclusion: The results reveal that an ethylacetate fraction of Zanthoxylum acanthopodium DC. fruits provides effective antimigration effects. Further studies are now planned to assess the potential of the ethylacetate fraction to inhibit angiogenesis in breast cancer and determine underlying mechanisms.
本研究旨在探讨刺花椒对4T1乳腺癌细胞系的抗迁移活性。方法：采用浸渍法，用正己烷和乙酸乙酯溶剂提取刺花椒果粉。使用MTT法评估细胞毒性和增殖情况，通过流式细胞术分析细胞周期。此外，采用显微镜法进行伤口愈合试验，并使用qRT-PCR测定COX-2和VEGFR-2的表达。结果：乙酸乙酯部位（EAF）的IC50为48.1±1.06μg/mL。在10μg/mL浓度下培养72小时后，EAF处理的活细胞数量为62.3±0.28%，细胞在G2-M期积累，在伤口愈合试验中抑制细胞迁移，COX-2（0.62±0.01）和VEGFR-2（0.39±0.003）的表达降低。结论：结果表明，刺花椒果实的乙酸乙酯部位具有有效的抗迁移作用。目前计划进一步研究评估乙酸乙酯部位抑制乳腺癌血管生成的潜力，并确定其潜在机制。
