Kunte Mugdha, Desai Krutika
Department of Biological Sciences, SD School of Science, SVKM's NMIMS (Deemed to be) University, Vile Parle (W), Mumbai, 400056, India.
Department of Microbiology, SVKM's Mithibai College, Vile Parle (W), Mumbai, 400056, India.
Protein J. 2017 Jun;36(3):186-195. doi: 10.1007/s10930-017-9707-0.
Spirulina platensis :have been studied for several biological activities. In the current study C-phycocyanin containing protein extract (C-PC extract) of Spirulina platensis have been studied for its effect on human matrix metalloproteinases (MMP-1, MMP-2 and MMP-9) and tissue inhibitors of MMPs (TIMP-1 and TIMP-2). In the present study, breast cancer cell line (MDA-MB 231) and hepatocellular cancer cell line (HepG2) were examined for inhibition of MMPs at different levels of expression after C-PC extract treatment. Herein, we have demonstrated that C-PC extract significantly reduced activity of MMP-2 by 55.13% and MMP-9 by 57.9% in HepG2 cells at 15 μg concentration. Additionally, the treatment has reduced mRNA expression of MMP-2 and MMP-9 at 20 μg concentration by 1.65-folds and 1.66-folds respectively. The C-PC extract treatment have also downregulated a mRNA expression of TIMP-2 by 1.12 folds at 20 μg concentration in HepG2 cells. Together, these results indicate that C-PC, extract successfully inhibited MMP-2 and -9 at different levels of expression and TIMP-2 at a mRNA expression level; however, extract did not have any effect on MMP-1 expressed in MDA-MB231 and TIMP-1 expressed in HepG2 cells as well as the exact mechanism of inhibition of MMP-2, MMP-9 and TIMP-2 remained unclear.
已针对多种生物活性进行了研究。在当前研究中，对钝顶螺旋藻含C-藻蓝蛋白的蛋白质提取物（C-PC提取物）对人基质金属蛋白酶（MMP-1、MMP-2和MMP-9）以及基质金属蛋白酶组织抑制剂（TIMP-1和TIMP-2）的影响进行了研究。在本研究中，检测了乳腺癌细胞系（MDA-MB 231）和肝癌细胞系（HepG2）在C-PC提取物处理后不同表达水平下基质金属蛋白酶的抑制情况。在此，我们已证明，在15μg浓度下，C-PC提取物可使HepG2细胞中MMP-2的活性显著降低55.13%，MMP-9的活性显著降低57.9%。此外，在20μg浓度下，该处理分别使MMP-2和MMP-9的mRNA表达降低了1.65倍和1.66倍。在20μg浓度下，C-PC提取物处理还使HepG2细胞中TIMP-2的mRNA表达下调了1.12倍。总之，这些结果表明，C-PC提取物在不同表达水平上成功抑制了MMP-2和-9，并在mRNA表达水平上抑制了TIMP-2；然而，提取物对MDA-MB231中表达的MMP-1和HepG2细胞中表达的TIMP-1没有任何影响，并且MMP-2、MMP-9和TIMP-2的具体抑制机制仍不清楚。
