Li Hua
College of Food Science and Technology, Henan University of Technology, Zhengzhou, 450001 China.
Chem Cent J. 2017 Mar 16;11:24. doi: 10.1186/s13065-017-0252-x. eCollection 2017.
(Pers.) Ricken, a member of the family Clavariaceae, has been widely prescribed for anti-aging and improving immunity. To extract and purify the polysaccharides, the main constituent of the fruiting-body, from and explore antioxidant activities was great significant.
polysaccharides (RBP) was extracted with water at 88.47 °C for 1.42 h with a solution to sample ratio of 10.94 mL g employing response surface methodology. Four purified fractions, RBP-1, RBP-2, RBP-3, and RBP-4, were obtained from column chromatography of DEAE-52 and Sephadex G-100. Among these four purified fractions, RBP-1, RBP-2, RBP-4 were mainly composed of glucose, while RBP-3 contained 41.36% mannose and 28.96% glucose. The molecular weights of RBP-1, RBP-2, RBP-3 and RBP-4 were 6.48, 36.12, 96.72 and 8.34 kDa, respectively. These four fractions are also tested for antioxidant activities in vitro, RBP-4 exhibited strong assay of reducing power and high scavenging activity on DPPH radical, while RBP-3 showed the stronger ability of hydroxyl radical scavenging activity.
Response surface methodology was successfully applied to optimize the ultrasonic extraction of polysaccharides from . RBP is an efficient natural antioxidant.
（平菇属）瑞氏木霉是珊瑚菌科的一员，已被广泛用于抗衰老和提高免疫力。从其子实体的主要成分中提取和纯化多糖并探索其抗氧化活性具有重要意义。
采用响应面法，在88.47℃下以10.94 mL/g的料液比用水提取多糖1.42小时。通过DEAE - 52和Sephadex G - 100柱色谱获得了四个纯化级分，即RBP - 1、RBP - 2、RBP - 3和RBP - 4。在这四个纯化级分中，RBP - 1、RBP - 2、RBP - 4主要由葡萄糖组成，而RBP - 3含有41.36%的甘露糖和28.96%的葡萄糖。RBP - 1、RBP - 2、RBP - 3和RBP - 4的分子量分别为6.48、36.12、96.72和8.34 kDa。还对这四个级分进行了体外抗氧化活性测试，RBP - 4表现出较强的还原力测定和对DPPH自由基的高清除活性，而RBP - 3表现出较强的羟基自由基清除能力。
响应面法成功应用于优化瑞氏木霉多糖的超声提取。RBP是一种高效的天然抗氧化剂。
