Department of Oral and Maxillofacial Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
School of Chemical Engineering and Technology, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150001, China.
Carbohydr Polym. 2017 Jun 1;165:30-38. doi: 10.1016/j.carbpol.2017.02.023. Epub 2017 Feb 7.
Sponges composed of different levels of composite collagen/oxidized microcrystalline cellulose (collagen/OMCC), denoted M1-M4, were studied to improve the hemostatic effect of single-collagen sponges. Surface morphological observations showed that structural combinations and intermolecular interactions occurred between collagen and OMCC in the composites. M2 presented the best physical properties and platelet activation and was thus selected for the investigations of the in vitro coagulation time and hemostatic and biological effects on animals. The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05). These findings were used to evaluate the hemostatic mechanism of M2, which can promote blood absorption and platelet activation and could be directly involved in the intrinsic coagulation pathway to accelerate hemostasis. Furthermore, M2 was not cytotoxic and was completely biodegraded in subcutaneous tissue within 28days.
研究了由不同层次的复合胶原/氧化微晶纤维素（胶原/OMCC）组成的海绵，记为 M1-M4，以提高单一胶原海绵的止血效果。表面形态观察表明，在复合材料中胶原和 OMCC 之间发生了结构组合和分子间相互作用。M2 表现出最佳的物理性能和血小板激活，因此被选择用于体外凝血时间和止血以及动物体内的生物效应的研究。结果表明，M2 可以缩短活化部分凝血活酶时间（APTT）和凝血酶时间（TT），在两种损伤模型中均表现出快速止血效果（P<0.05）。这些发现用于评估 M2 的止血机制，它可以促进血液吸收和血小板激活，并可直接参与内源性凝血途径加速止血。此外，M2 无细胞毒性，在 28 天内在皮下组织中完全降解。
