Austin P J, Flemington E, Yandava C N, Strominger J L, Speck S H
Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115.
Proc Natl Acad Sci U S A. 1988 Jun;85(11):3678-82. doi: 10.1073/pnas.85.11.3678.
Several cDNA clones containing the Epstein-Barr virus BamHI fragment H rightward open reading frame 1 (BHRF1) have been recovered from the tightly latent lymphoblastoid cell line IB4. These clones contain the 5' leader exons encoded in the major internal repeat 1 and the viral BamHI fragment Y, identified in the rightwardly transcribed viral mRNAs associated with the latent viral life cycle. In addition, a cDNA clone containing BHRF1 from the Burkitt lymphoma cell line Jijoye was also recovered and exhibits a distinctive splicing pattern. In vitro transcription and translation of BHRF1, followed by immunoprecipitation with Epstein-Barr virus-positive human sera, indicates that this viral antigen is expressed during infection. RNA blot analyses with a wide panel of lymphoblastoid and Burkitt lymphoma cell lines revealed a complex pattern of transcription. Hybridization data obtained with several probes is presented.
从紧密潜伏的淋巴母细胞系IB4中已获得了几个包含爱泼斯坦-巴尔病毒BamHI片段H右向开放阅读框1（BHRF1）的cDNA克隆。这些克隆包含在主要内部重复序列1中编码的5'前导外显子以及病毒BamHI片段Y，该片段在与潜伏病毒生命周期相关的右向转录病毒mRNA中被鉴定出来。此外，还从伯基特淋巴瘤细胞系Jijoye中获得了一个包含BHRF1的cDNA克隆，它表现出独特的剪接模式。对BHRF1进行体外转录和翻译，然后用爱泼斯坦-巴尔病毒阳性的人血清进行免疫沉淀，结果表明这种病毒抗原在感染期间表达。用多种淋巴母细胞系和伯基特淋巴瘤细胞系进行的RNA印迹分析揭示了一种复杂的转录模式。展示了用几种探针获得的杂交数据。
