Kim Soo Jeong, Jang Ye Won, Hyung Kyung Eun, Lee Da Kyoung, Hyun Kee Hyeob, Jeong Seung Hwarn, Min Kyung Hoon, Kang Wonku, Jeong Ji Hoon, Park So-Young, Hwang Kwang Woo
College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.
Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul, Republic of Korea.
Bioelectromagnetics. 2017 Jul;38(5):374-385. doi: 10.1002/bem.22049. Epub 2017 Mar 29.
In recent years, there has been a dramatic increase in the number and variety of electronic devices that emit electromagnetic waves. Because people live and work in close proximity to these pieces of electrical equipment, there is growing concern surrounding the destruction of homeostasis by electromagnetic field exposure. In the present study, the effects of 60 Hz 0.8 mT extremely low-frequency electromagnetic fields (ELF-EMF) on a macrophage cell line (RAW 264.7) were examined. Under defined ELF-EMF exposure conditions, the production of nitric oxide and pro-inflammatory cytokines, TNF-α, IL-1β, and IL-6, were increased in RAW 264.7 cells and the expression of those genes was also upregulated. However, cell proliferation was not altered. Translocation of NF-κB (nuclear factor kappa B), molecules that act downstream of the pro-inflammatory cytokines, were increased to the nucleus under ELF-EMF exposure conditions. In addition, we found that ELF-EMF exposure elevated activation of nuclear factor of activated T cells (NFAT) 2, as well as positively affected the influx of calcium. Furthermore, with both the presence of a potent antioxidant (Resveratrol) and downregulation of the antioxidant-related gene Prx-1 (Peroxiredoxin-1), ELF-EMF was associated with higher inflammatory responses of macrophages. These results suggest that an ELF-EMF amplifies inflammatory responses through enhanced macrophage activation and can decrease the effectiveness of antioxidants. Bioelectromagnetics. 38:374-385, 2017. © 2017 Wiley Periodicals, Inc.
近年来，发射电磁波的电子设备在数量和种类上都急剧增加。由于人们在这些电气设备附近生活和工作，电磁场暴露对体内平衡的破坏日益受到关注。在本研究中，检测了60Hz 0.8mT极低频电磁场（ELF-EMF）对巨噬细胞系（RAW 264.7）的影响。在确定的ELF-EMF暴露条件下，RAW 264.7细胞中一氧化氮和促炎细胞因子TNF-α、IL-1β和IL-6的产生增加，且这些基因的表达也上调。然而，细胞增殖未发生改变。在ELF-EMF暴露条件下，作为促炎细胞因子下游作用分子的核因子κB（NF-κB）向细胞核的转位增加。此外，我们发现ELF-EMF暴露提高了活化T细胞核因子（NFAT）2的激活水平，并对钙内流产生积极影响。此外，在强效抗氧化剂（白藜芦醇）存在以及抗氧化相关基因Prx-1（过氧化物酶1）下调的情况下，ELF-EMF与巨噬细胞更高的炎症反应相关。这些结果表明，ELF-EMF通过增强巨噬细胞活化来放大炎症反应，并可降低抗氧化剂的有效性。《生物电磁学》。38:374 - 385，2017年。©2017威利期刊公司。
