Elmendorf S, McSharry J, Laffin J, Fogleman D, Lehman J M
Department of Microbiology, Albany Medical College, New York 12208.
Cytometry. 1988 May;9(3):254-60. doi: 10.1002/cyto.990090311.
An early cytomegalovirus (CMV) antigen was detected with a monoclonal antibody by two-color fluorescent flow cytometry. With the aid of a human diploid fibroblast cell strain, FLOW 2000, infected with the AD169 strain of CMV, the viral antigen and the DNA content of infected or uninfected cells were measured. There was no evidence of change in the cell-cycle distribution of the infected cells. The viral antigen was detected within 30 minutes following virus adsorption at 0.1 and 1.0 plaque-forming units/cells; and the percentage of positive cells increased with time and viral dosage. All stages of the cell cycle were susceptible to viral infection and the average fluorescence was greater than the background fluorescence. Flow cytometry detected the viral antigen earlier than conventional immunofluorescent microscopy and cell culture for CMV cytopathological effect (CPE). Ten bronchoalveolar lavages assayed by flow cytometry and conventional diagnostic procedures demonstrated that flow cytometry might be useful in early diagnosis for CMV infection.
通过双色荧光流式细胞术，用单克隆抗体检测到一种早期巨细胞病毒（CMV）抗原。借助感染了CMV AD169株的人二倍体成纤维细胞系FLOW 2000，测量了感染或未感染细胞的病毒抗原和DNA含量。未发现感染细胞的细胞周期分布有变化。在病毒以0.1和1.0蚀斑形成单位/细胞吸附后30分钟内检测到病毒抗原；阳性细胞百分比随时间和病毒剂量增加。细胞周期的所有阶段都易受病毒感染，平均荧光强度大于背景荧光。流式细胞术比传统免疫荧光显微镜和细胞培养检测CMV细胞病变效应（CPE）更早地检测到病毒抗原。通过流式细胞术和传统诊断程序对10份支气管肺泡灌洗样本进行检测表明，流式细胞术可能有助于CMV感染的早期诊断。
