McSharry J J, Costantino R, McSharry M B, Venezia R A, Lehman J M
Department of Microbiology and Immunology, Albany Medical Center, New York 12208.
J Clin Microbiol. 1990 Aug;28(8):1864-6. doi: 10.1128/jcm.28.8.1864-1866.1990.
Murine monoclonal antibodies (MAbs) against herpes simplex virus type 1 and 2 (HSV-1 and -2, respectively) nuclear antigens were used to identify cells infected with HSV-1 or -2 by indirect immunofluorescence in conjunction with flow cytometry after virus amplification of MRC-5 cell monolayers. The results indicate that MAbs Q1, Q2, and H-640 detect HSV-1- and HSV-2-infected cells, MAb SD-1 detects HSV-2- but not HSV-1-infected cells, and MAb 58-S detects HSV-1- but not HSV-2-infected cells. MAb Q1, which detects HSV-1- as well as HSV-2-infected cells, was used to detect HSV-infected cells after inoculation and overnight (16- to 20-h) incubation of MRC-5 monolayers with clinical samples suspected of containing HSV. In comparing the efficiency of flow cytometry with cytopathic effect (CPE) in tissue culture for detecting HSV in clinical samples, HSV was detected by flow cytometry in 77% of the cases where HSV was detected by CPE in tissue culture. In many cases, flow cytometry detected HSV from 1 to 3 days before HSV was detected by CPE.
利用抗单纯疱疹病毒1型和2型（分别为HSV-1和HSV-2）核抗原的鼠单克隆抗体（MAb），在MRC-5细胞单层进行病毒扩增后，通过间接免疫荧光结合流式细胞术来鉴定感染HSV-1或HSV-2的细胞。结果表明，单克隆抗体Q1、Q2和H-640可检测出感染HSV-1和HSV-2的细胞，单克隆抗体SD-1可检测出感染HSV-2但未感染HSV-1的细胞，单克隆抗体58-S可检测出感染HSV-1但未感染HSV-2的细胞。单克隆抗体Q1可检测出感染HSV-1以及HSV-2的细胞，在将MRC-5单层细胞与疑似含有HSV的临床样本接种并孵育过夜（16至20小时）后，用其检测感染HSV的细胞。在比较流式细胞术与组织培养中细胞病变效应（CPE）检测临床样本中HSV的效率时，在组织培养中通过CPE检测出HSV的病例中，有77%通过流式细胞术检测出了HSV。在许多情况下，流式细胞术在通过CPE检测出HSV前1至3天就检测到了HSV。
