姜科植物根茎蛋白水解物的抗氧化和抗增殖活性

Antioxidant and antiproliferative activities of protein hydrolysate from the rhizomes of Zingiberaceae plants.

作者信息

Inthuwanarud Kanok, Sangvanich Polkit, Puthong Songchan, Karnchanatat Aphichart

机构信息

Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok, Thailand.

The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok, Thailand.

出版信息

Pak J Pharm Sci. 2016 Nov;29(6):1893-1900.

PMID:28375103

Abstract

Plant proteins have been investigated for their antioxidant activities, but there are still no reports detailing the antioxidant activity levels of plants in the Zingiberaceae family, which are popular food agents and used in folklore medicine. In this study, the crude rhizome protein extract and associated pepsin/pancreatin protein hydrolysate of 15 plants in the Zingiberaceae family were screened using the DPPH method for antioxidant activity. The protein hydrolysate of C. zedoaria possessed the highest antioxidant activity (ICof 25.7±6.3µg/mL), which was close to that of the reference ascorbic acid (ICof 22.3±1.8µg/mL). After enrichment by Q Sepharose ion exchange chromatography using a five step elution gradient of increasing NaCl concentration (0, 0.25, 0.5, 0.75 and 1M), the fraction eluting in the 0.5M NaCl (F50) showed the highest antioxidant activity (IC of 41.78±2.9µg/mL), and was found to have weak in vitro cytotoxicity against the HEP-G2 and SW620 cell lines (IC of 200.8±11.8 and 241.0±9.3µg/mL, respectively), but not the BT474, CHAGO and KATO-3 cell lines. F50 had an estimated molecular weight by MALDI-TOF mass spectrometry of 12,400-12,800 Da.

摘要

植物蛋白的抗氧化活性已得到研究，但对于姜科植物的抗氧化活性水平仍未有详细报道，姜科植物是常见的食用植物且在民间医学中有所应用。在本研究中，采用DPPH法对15种姜科植物的根茎粗蛋白提取物及相关的胃蛋白酶/胰蛋白酶蛋白水解产物进行抗氧化活性筛选。莪术的蛋白水解产物具有最高的抗氧化活性（IC50为25.7±6.3μg/mL），接近参考物质抗坏血酸的抗氧化活性（IC50为22.3±1.8μg/mL）。使用NaCl浓度递增的五步洗脱梯度（0、0.25、0.5、0.75和1M）通过Q Sepharose离子交换色谱进行富集后，在0.5M NaCl中洗脱的组分（F50）表现出最高的抗氧化活性（IC50为41.78±2.9μg/mL），并且发现其对HEP-G2和SW620细胞系具有较弱的体外细胞毒性（IC50分别为200.8±11.8和241.0±9.3μg/mL），但对BT474、CHAGO和KATO-3细胞系无细胞毒性。通过基质辅助激光解吸电离飞行时间质谱法测定，F50的估计分子量为12,400 - 12,800 Da。