多种序列特异性转录因子在体外调节巨细胞病毒增强子活性。
Multiple sequence-specific transcription factors modulate cytomegalovirus enhancer activity in vitro.
作者信息
Ghazal P, Lubon H, Hennighausen L
机构信息
Laboratory of Biochemistry and Metabolism, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
出版信息
Mol Cell Biol. 1988 Apr;8(4):1809-11. doi: 10.1128/mcb.8.4.1809-1811.1988.
Abstract
The possibility of DNA-binding proteins interacting in vitro with the polymerase II transcriptional machinery was explored by using a competition assay with individual target sequences for enhancer-binding factors. Transcription factors binding to at least five specific enhancer sequences mediate the activity of the human cytomegalovirus immediate-early 1 gene in vitro. Furthermore, our data suggest that individual DNA-bound enhancer factors can interact with the promoter transcription complex.
摘要
通过使用针对增强子结合因子的单个靶序列进行竞争试验，探索了DNA结合蛋白在体外与聚合酶II转录机制相互作用的可能性。与至少五个特定增强子序列结合的转录因子在体外介导人巨细胞病毒立即早期1基因的活性。此外，我们的数据表明，单个与DNA结合的增强子因子可以与启动子转录复合物相互作用。
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