Fukushima S, Tamano S, Shibata M A, Kurata Y, Hirose M, Ito N
First Department of Pathology, Nagoya City University Medical School, Japan.
Carcinogenesis. 1988 Jul;9(7):1203-6. doi: 10.1093/carcin/9.7.1203.
The promoting activities of NaHCO3 and NaCl in two-stage urinary bladder carcinogenesis in rats initiated with N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) were investigated. Male F344 rats were given 0.05% BBN in their drinking water for 4 weeks and then treated with basal diet containing either 3% NaHCO3, 1% NaCl or no added chemical for 32 weeks. NaHCO3 significantly increased the induction of neoplastic and preneoplastic lesions of the urinary bladder, whereas NaCl did not. NaHCO3 produced elevation of urinary pH and urinary Na+ concentration. NaCl increased urinary Na+ concentration without the elevation of urinary pH. In an additional experiment, DNA synthesis in the urinary bladder epithelium was significantly increased in the groups given 3% NaHCO3, 5% sodium L-ascorbate and 1% NaCl. These results confirm that urinary components, increase in urinary pH and Na+ concentration play an important role in the promotion of urinary bladder carcinogenesis in rats.
研究了碳酸氢钠（NaHCO3）和氯化钠（NaCl）对用N-丁基-N-（4-羟丁基）亚硝胺（BBN）启动的大鼠两阶段膀胱致癌作用的促进活性。给雄性F344大鼠饮用含0.05%BBN的水4周，然后用含3%NaHCO3、1%NaCl或不添加化学物质的基础饲料处理32周。NaHCO3显著增加了膀胱肿瘤性和癌前病变的诱导，而NaCl则没有。NaHCO3导致尿液pH值和尿Na+浓度升高。NaCl增加了尿Na+浓度，但没有使尿液pH值升高。在另一项实验中，给予3%NaHCO3、5%L-抗坏血酸钠和1%NaCl的组中，膀胱上皮细胞的DNA合成显著增加。这些结果证实，尿液成分、尿液pH值和Na+浓度的增加在大鼠膀胱致癌作用的促进中起重要作用。
