Alcaín F J, Burón M I
Departamento de Biología Celular, Facultad de Ciencias, Universidad de Córdoba, Spain.
J Bioenerg Biomembr. 1994 Aug;26(4):393-8. doi: 10.1007/BF00762780.
Ascorbate, an essential nutrient in humans, primates, and guinea pig, is involved in many cellular functions. Ascorbate also modulates cell growth and differentiation. Ascorbate can reduce or stimulate the growth of tumor cells, depending on the cell type. The inhibitory effect is not specific for the biological active isomer L-ascorbate, and isoascorbate and D-ascorbate are more effective in reducing cell growth than L-ascorbate. These results indicate that ascorbate has a cytotoxic effect by killing cells directly, rather a cytostatic one. However, only L-ascorbate is able to stimulate cell growth, but the mechanism of this stimulation is still unknown. L-Ascorbate stimulates the in vitro differentiation of several mesenchyme-derived cell types by altering the expression of multiple genes as the cell progresses through specific differentiation programs. Stimulation of collagen matrix at gene transcription, mRNA stabilization, hydroxylation, and secretion is a key role for L-ascorbate. L-Ascorbate also prevents cell transformation by stabilization of the differentiated state and cooperates with other agents to induce differentiation in a leukemia cell line.
抗坏血酸是人类、灵长类动物和豚鼠必需的营养素，参与许多细胞功能。抗坏血酸还调节细胞生长和分化。抗坏血酸可根据细胞类型减少或刺激肿瘤细胞的生长。这种抑制作用并非生物活性异构体L-抗坏血酸所特有，异抗坏血酸和D-抗坏血酸在抑制细胞生长方面比L-抗坏血酸更有效。这些结果表明，抗坏血酸通过直接杀死细胞具有细胞毒性作用，而不是细胞抑制作用。然而，只有L-抗坏血酸能够刺激细胞生长，但其刺激机制仍不清楚。L-抗坏血酸通过改变多个基因的表达，在细胞通过特定分化程序时刺激几种间充质来源细胞类型的体外分化。在基因转录、mRNA稳定、羟基化和分泌过程中刺激胶原蛋白基质是L-抗坏血酸的关键作用。L-抗坏血酸还通过稳定分化状态防止细胞转化，并与其他因子协同作用诱导白血病细胞系分化。
