Bachtiar Endang W, Amir Lisa Rinanda, Suhardi Pradono, Abas Basril
Department of Oral Biology, Faculty of Dentistry and Oral Sciences Research Center, Universitas Indonesia , Jakarta, Indonesia.
Department of Oral Surgery, Faculty of Dentistry, Universitas Indonesia , Jakarta, Indonesia.
Interv Med Appl Sci. 2016 Jun 1;8(2):77-81. doi: 10.1556/1646.8.2016.2.5.
To examine the degradation of three scaffolds composed of hydroxyapatite/tricalcium phosphate (HA/TCP) with 70∶30 ratio, HA/TCP with 50∶50 ratio, and HA/TCP/chitosan scaffold as analyzed by the RNA expression of matrix metalloprotease 2 (MMP2), interleukin 13 (IL13), and tartrate-resistant acid phosphatase (TRAP) genes.
The three tested scaffolds and dental pulp stromal cells (DPSCs) were transplanted into the mandibular bone defect of six young male . Defect on the left mandible served as the experimental group and the right mandible served as control group (split mouth design). The biopsies were retrieved at 0, 2, and 4 weeks after cell-scaffold transplantation. The expression of MMP2, IL13, and TRAP was analyzed by real-time PCR (RT-PCR).
The inflammatory cells were still detected in areas where active bone and blood vessel formation occurred. The remnants of scaffold biomaterials were rarely seen. The expression of MMP2, IL13, and TRAP was observed in all samples. Their expressions were increased at week 4 and the decrease of TRAP gene expression in the experimental group was found higher than the control group. TRAP gene in the HA/TCP/chitosan group was found to be the highest at week 2 and lowest at week 4.
Degradation of the scaffold did not induce higher inflammatory response compared to the control yet it induced more osteoclast activity.
通过分析基质金属蛋白酶2（MMP2）、白细胞介素13（IL13）和抗酒石酸酸性磷酸酶（TRAP）基因的RNA表达，研究70∶30比例的羟基磷灰石/磷酸三钙（HA/TCP）支架、50∶50比例的HA/TCP支架以及HA/TCP/壳聚糖支架的降解情况。
将三种受试支架和牙髓基质细胞（DPSCs）移植到六只年轻雄性大鼠的下颌骨缺损处。左侧下颌骨缺损作为实验组，右侧下颌骨作为对照组（双侧对照设计）。在细胞 - 支架移植后0、2和4周取出活检组织。通过实时聚合酶链反应（RT-PCR）分析MMP2、IL13和TRAP的表达。
在有活跃骨和血管形成的区域仍可检测到炎性细胞。很少见到支架生物材料的残余物。在所有样本中均观察到MMP2、IL13和TRAP的表达。它们的表达在第4周增加，并且发现实验组中TRAP基因表达的下降高于对照组。发现HA/TCP/壳聚糖组中的TRAP基因在第2周时最高，在第4周时最低。
与对照组相比，支架的降解并未引发更高的炎症反应，但却诱导了更多的破骨细胞活性。
