Owusu Enid, Newman Mercy J, Addo Kwesi K, Addo Phyllis
Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana.
Department of Medical Microbiology, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana.
Can J Infect Dis Med Microbiol. 2017;2017:5180984. doi: 10.1155/2017/5180984. Epub 2017 Mar 14.
The current definitive treatment of Buruli ulcer with antibiotics makes the issue of antimicrobial drug resistance an unavoidable one. This is as a result of drug misuse by health personnel and patients' noncompliance to treatment regimen. Monitoring of these factors and screening for new effective antimicrobials are crucial to effective management of Buruli ulcer disease. This study therefore investigated the inhibitory activity of some antibiotics against isolates of . Activity of eight antibiotics was tested against twelve isolates (2 reference strains and 10 clinical isolates). The anti- activities were determined by the agar dilution method and the minimum inhibitory concentrations (MICs) were determined by the agar proportion method. All antimicrobials investigated had activity against isolates tested. The MICs ranged from 0.16 g/mL to 2.5 g/mL. Azithromycin recorded the highest inhibitory activity at a mean MIC of 0.39 g/mL, whilst clofazimine a second-line antileprosy drug, recorded the lowest at a mean MIC of 2.19 g/mL. Among the four antituberculosis drugs, rifampicin had the highest activity with a mean MIC of 0.81 g/mL. Azithromycin could be considered as a lucrative alternative to existing treatment methods for inhibiting in Ghana.
目前用抗生素对布鲁里溃疡进行的确定性治疗使得抗菌药物耐药性问题不可避免。这是卫生人员药物使用不当以及患者不遵守治疗方案所致。监测这些因素并筛选新的有效抗菌药物对于有效管理布鲁里溃疡病至关重要。因此，本研究调查了某些抗生素对……分离株的抑制活性。针对12株……分离株（2株参考菌株和10株临床分离株）测试了8种抗生素的活性。通过琼脂稀释法测定抗菌活性，通过琼脂比例法测定最低抑菌浓度（MIC）。所有研究的抗菌药物对所测试的……分离株均有活性。MIC范围为0.16μg/mL至2.5μg/mL。阿奇霉素的抑制活性最高，平均MIC为0.39μg/mL，而二线抗麻风病药物氯法齐明的抑制活性最低，平均MIC为2.19μg/mL。在四种抗结核药物中，利福平的活性最高，平均MIC为0.81μg/mL。在加纳，阿奇霉素可被视为抑制……的现有治疗方法的一种有利替代方案。
