Cryer Matthew, Lane Kyle, Greer Mary, Cates Rex, Burt Scott, Andrus Merritt, Zou Jiping, Rogers Paul, Hansen Marc D H, Burgado Jillybeth, Panayampalli Subbian Satheshkumar, Day Craig W, Smee Donald F, Johnson Brent F
a Department of Microbiology and Molecular Biology , Brigham Young University , Provo , UT , USA.
b Department of Biology , Brigham Young University , Provo , UT , USA.
Pharm Biol. 2017 Dec;55(1):1586-1591. doi: 10.1080/13880209.2017.1310907.
Kalanchoe pinnata (Lam.) Pers. (Crassulaceae) is a succulent plant that is known for its traditional antivirus and antibacterial usage.
This work examines two compounds identified from the K. pinnata plant for their antivirus activity against human alphaherpesvirus (HHV) 1 and 2 and vaccinia virus (VACV).
Compounds KPB-100 and KPB-200 were isolated using HPLC and were identified using NMR and MS. Both compounds were tested in plaque reduction assay of HHV-2 wild type (WT) and VACV. Both compounds were then tested in virus spread inhibition and virus yield reduction (VYR) assays of VACV. KPB-100 was further tested in viral cytopathic effect (CPE) inhibition assay of HHV-2 TK-mutant and VYR assay of HHV-1 WT.
KPB-100 and KPB-200 inhibited HHV-2 at IC values of 2.5 and 2.9 μg/mL, respectively, and VACV at IC values of 3.1 and 7.4 μg/mL, respectively, in plaque reduction assays. In virus spread inhibition assay of VACV KPB-100 and KPB-200 yielded IC values of 1.63 and 13.2 μg/mL, respectively, and KPB-100 showed a nearly 2-log reduction in virus in VYR assay of VACV at 20 μg/mL. Finally, KPB-100 inhibited HHV-2 TK- at an IC value of 4.5 μg/mL in CPE inhibition assay and HHV-1 at an IC of 3.0 μg/mL in VYR assay.
Both compounds are promising targets for synthetic optimization and in vivo study. KPB-100 in particular showed strong inhibition of all viruses tested.
落地生根（景天科）是一种肉质植物，以其传统的抗病毒和抗菌用途而闻名。
本研究检测了从落地生根植物中鉴定出的两种化合物对人α疱疹病毒（HHV）1和2以及痘苗病毒（VACV）的抗病毒活性。
使用高效液相色谱法分离化合物KPB - 100和KPB - 200，并使用核磁共振和质谱进行鉴定。两种化合物均在HHV - 2野生型（WT）和VACV的蚀斑减少试验中进行测试。然后，两种化合物均在VACV的病毒传播抑制和病毒产量降低（VYR）试验中进行测试。KPB - 100在HHV - 2 TK突变体的病毒细胞病变效应（CPE）抑制试验和HHV - 1 WT的VYR试验中进一步测试。
在蚀斑减少试验中，KPB - 100和KPB - 200分别以2.5和2.9μg/mL的IC值抑制HHV - 2，以3.1和7.4μg/mL的IC值抑制VACV。在VACV的病毒传播抑制试验中，KPB - 100和KPB - 200的IC值分别为1.63和13.2μg/mL，并且在20μg/mL的VACV的VYR试验中，KPB - 100显示病毒减少近2个对数。最后，在CPE抑制试验中，KPB - 100以4.5μg/mL的IC值抑制HHV - 2 TK - ，在VYR试验中以3.0μg/mL的IC值抑制HHV - 1。
两种化合物都是合成优化和体内研究的有前景的靶点。特别是KPB - 100对所有测试病毒均表现出强烈抑制作用。
