Rosa Antonella, Maxia Andrea, Putzu Danilo, Atzeri Angela, Era Benedetta, Fais Antonella, Sanna Cinzia, Piras Alessandra
Department of Biomedical Sciences, University of Cagliari, Cittadella Universitaria, SS 554, Km 4.5, 09042 Monserrato, CA, Italy.
Department of Life and Environmental Sciences, University of Cagliari, Viale Sant'Ignazio da Laconi 13, 09123 Cagliari, Italy.
Food Chem. 2017 Sep 1;230:82-90. doi: 10.1016/j.foodchem.2017.03.019. Epub 2017 Mar 6.
We studied the total phenols and flavonoids, liposoluble antioxidants, fatty acid and triacylglycerol profiles, and oxidative status of oil obtained from Lycium europaeum fruits following supercritical CO extraction (at 30MPa and 40°C). Linoleic (52%), palmitic (18%), oleic (13%), and α-linolenic (6%) were the main oil fatty acids, while trilinolein and palmitodilinolein/oleodilinolein represented the main triacylglycerols. The oil was characterized by high levels of all-trans-zeaxanthin and all-trans-β-carotene (755 and 332μg/g of oil, respectively), α-tocopherol (308μg/g of oil), total phenols (13.6mg gallic acid equivalents/g of oil), and total flavonoids (6.8mg quercetin equivalents/g of oil). The oil showed radical scavenging activities (ABTS and DPPH assays) and inhibited Caco-2 cell growth. Moreover, the incubation of differentiated Caco-2 cells with a non-toxic oil concentration (100μg/mL) induced a significant intracellular accumulation of essential fatty acids. The results qualify L. europaeum oil as a potential source for food/pharmaceutical applications.
我们研究了经超临界CO₂萃取（30MPa，40°C）得到的欧洲枸杞果实油中的总酚和黄酮类化合物、脂溶性抗氧化剂、脂肪酸和三酰甘油谱以及氧化状态。亚油酸（52%）、棕榈酸（18%）、油酸（13%）和α-亚麻酸（6%）是主要的油脂肪酸，而三亚油酸甘油酯和棕榈二亚油酸甘油酯/油二亚油酸甘油酯是主要的三酰甘油。该油的特征在于含有高水平的全反式玉米黄质和全反式β-胡萝卜素（分别为755和332μg/g油）、α-生育酚（308μg/g油）、总酚（13.6mg没食子酸当量/g油）和总黄酮（6.8mg槲皮素当量/g油）。该油具有自由基清除活性（ABTS和DPPH测定）并抑制Caco-2细胞生长。此外，用无毒油浓度（100μg/mL）孵育分化的Caco-2细胞会导致必需脂肪酸在细胞内显著积累。这些结果表明欧洲枸杞油是食品/制药应用的潜在来源。
