Nakamura Yuko, Nagaya Tadanobu, Sato Kazuhide, Okuyama Shuhei, Ogata Fusa, Wong Karen, Adler Stephen, Choyke Peter L, Kobayashi Hisataka
Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland; and.
Clinical Research Directorate/Clinical Monitoring Research Program, Leidos Biomedical Research, Inc., National Cancer Institute Campus at Frederick, Frederick, Maryland.
J Nucl Med. 2017 Sep;58(9):1395-1400. doi: 10.2967/jnumed.116.188789. Epub 2017 Apr 13.
Near-infrared photoimmunotherapy (NIR-PIT) is a new cancer treatment that combines the specificity of antibodies for targeting tumors with toxicity induced by photoabsorbers after irradiation with NIR light. A limitation of NIR-PIT is the inability to deliver NIR light to a tumor located deep inside the body. Cerenkov radiation (CR) is the ultraviolet and blue light that is produced by a charged particle traveling through a dielectric medium faster than the speed of light in that medium and is commonly produced during radioactive decay. Here, we demonstrate the feasibility of using CR generated by F-FDG accumulated in tumors to induce photoimmunotherapy. Using A431-luc cells, we evaluated the therapeutic effects of CR-PIT in vitro and in vivo using bioluminescence imaging. CR-PIT showed significant suppression of tumor size, but the decrease of bioluminescence after CR-PIT was not observed consistently over the entire time course. Although CR-PIT can induce tumor killing deep within body, it is less effective than NIR-PIT, possibly related to the relatively lower efficiency of short wavelength light than NIR.
近红外光免疫疗法（NIR-PIT）是一种新型癌症治疗方法，它将抗体靶向肿瘤的特异性与近红外光照射后光吸收剂诱导的毒性相结合。NIR-PIT的一个局限性是无法将近红外光传递到位于身体深处的肿瘤。切伦科夫辐射（CR）是带电粒子在电介质中以高于该介质中光速的速度传播时产生的紫外线和蓝光，通常在放射性衰变过程中产生。在此，我们证明了利用肿瘤中积累的F-FDG产生的CR诱导光免疫疗法的可行性。使用A431-luc细胞，我们通过生物发光成像在体外和体内评估了CR-PIT的治疗效果。CR-PIT显示出对肿瘤大小的显著抑制，但在整个时间过程中未始终观察到CR-PIT后生物发光的降低。尽管CR-PIT可以在体内深处诱导肿瘤杀伤，但它比NIR-PIT效果差，这可能与短波长光相对于近红外光的效率相对较低有关。
