Immunomodulation Division, Defence Institute of Physiology and Allied Sciences, DRDO, Lucknow Road, Timarpur, Delhi, 110054, India.
Inflammopharmacology. 2018 Feb;26(1):291-301. doi: 10.1007/s10787-017-0345-0. Epub 2017 Apr 13.
The study explores the anti-inflammatory activity of components present in fractions obtained from leaves of Hippophae rhamnoides in mouse peritoneal macrophages.
Immunomodulators salvage the immune response by enhancing or reducing its capacity to the required level. Plant extracts are extensively used as immunomodulators because of their easy availability, simple methods of preparation and minimum side effects with maximum efficacy.
The present study was conducted to assess the immunomodulatory activities of phyto constituents present in Seabuckthorn leaves. The aqueous-alcoholic leaf extract was subjected to successive and parallel extraction in the presence of polar and non-polar solvents for fractionation of compounds. Based on the yield, three fractions were selected viz. parallel methanol (PM), successive chloroform (SC) and successive methanol (SM) and screened for in vitro immunomodulatory activities. Peritoneal macrophages were isolated from Balb/c mice and cultured with or without LPS to evaluate the immunomodulatory effect of the three fractions on cell viability, hemolytic activity, nitric oxide (NO) production, cytokine levels, iNOS and COX-2 expressions.
The results revealed that none of the three fractions induced hemolysis. Cells treated with PM fraction significantly suppressed LPS-induced NO production and pro-inflammatory cytokines such as TNF-α, IL-6 and IFN-γ as compared to SC and SM treatment. The iNOS and COX-2 expressions were also significantly reduced after treatment with PM fraction.
The decrease in LPS-induced NO production, pro-inflammatory cytokine secretion, iNOS and COX-2 expression signifies anti-inflammatory properties of PM fraction containing tannins, proteins and carbohydrate groups. Hence, this plant-derived immunomodulator can be used as a therapeutic agent in inflammatory diseases.
本研究探讨了沙棘叶提取物中存在的成分在小鼠腹腔巨噬细胞中的抗炎活性。
免疫调节剂通过增强或降低其能力来达到所需水平来挽救免疫反应。由于植物提取物易于获得、制备方法简单且副作用最小、疗效最大，因此被广泛用作免疫调节剂。
本研究旨在评估沙棘叶中存在的植物成分的免疫调节活性。将水醇叶提取物在极性和非极性溶剂存在下进行连续和并行提取，以对化合物进行分级。根据产率，选择了三个级分，即平行甲醇 (PM)、连续氯仿 (SC) 和连续甲醇 (SM)，并对其进行体外免疫调节活性筛选。从 Balb/c 小鼠中分离腹腔巨噬细胞，并与 LPS 一起培养或不培养，以评估三个级分对细胞活力、溶血活性、一氧化氮 (NO) 产生、细胞因子水平、iNOS 和 COX-2 表达的免疫调节作用。
结果表明，三个级分均未引起溶血。与 SC 和 SM 处理相比，PM 级分处理的细胞显著抑制了 LPS 诱导的 NO 产生和促炎细胞因子（如 TNF-α、IL-6 和 IFN-γ）的产生。用 PM 级分处理后，iNOS 和 COX-2 的表达也明显降低。
PM 级分中含有单宁、蛋白质和碳水化合物，可降低 LPS 诱导的 NO 产生、促炎细胞因子分泌、iNOS 和 COX-2 表达，表明其具有抗炎特性。因此，这种植物来源的免疫调节剂可用于炎症性疾病的治疗。
