Arrizabalaga Julien H, Nollert Matthias U
a Stephenson School of Biomedical Engineering, University of Oklahoma , Norman , OK , USA.
b School of Chemical, Biological & Materials Engineering, University of Oklahoma , Norman , OK , USA.
Adipocyte. 2017 Jul 3;6(3):217-223. doi: 10.1080/21623945.2017.1312040. Epub 2017 Mar 30.
Adipose-derived stem cells represent a reliable adult stem cell source thanks to their abundance, straightforward isolation, and broad differentiation abilities. Consequently, human adipose-derived stem cells (hASCs) have been used in vitro for several innovative cellular therapy and regenerative medicine applications. However, the translation of a novel technology from the laboratory to the clinic requires first to evaluate its safety, feasibility, and potential efficacy through preclinical studies in animals. The anatomy and physiology of pigs and humans are very similar, establishing pigs as an attractive and popular large animal model for preclinical studies. Knowledge of the properties of porcine adipose-derived stem cells (pASCs) used in preclinical studies is critical for their success. While hASCs have been extensively studied this past decade, only a handful of reports relate to pASCs. The aim of this concise review is to summarize the current findings about the isolation of pASCs, their culture, proliferation, and immunophenotype. The differentiation abilities of pASCs and their applications in porcine preclinical models will also be reported.
脂肪来源干细胞因其丰富性、分离简便性和广泛的分化能力，是一种可靠的成体干细胞来源。因此，人脂肪来源干细胞（hASCs）已在体外用于多种创新的细胞治疗和再生医学应用。然而，将一项新技术从实验室转化到临床，首先需要通过动物的临床前研究来评估其安全性、可行性和潜在疗效。猪和人的解剖学与生理学非常相似，这使猪成为临床前研究中一种有吸引力且常用的大型动物模型。了解临床前研究中使用的猪脂肪来源干细胞（pASCs）的特性对其成功至关重要。尽管在过去十年中hASCs已得到广泛研究，但关于pASCs的报道却寥寥无几。这篇简要综述的目的是总结目前关于pASCs的分离、培养、增殖和免疫表型的研究结果。还将报道pASCs的分化能力及其在猪临床前模型中的应用。
