Ohlsson Bodil, Roth Bodil, Larsson Ewa, Höglund Peter
Department of Internal Medicine, Skane University Hospital, Malmo, Sweden.
Department of Cardiothoracic Surgery, Lund University, Skane University Hospital, Lund, Sweden.
Biomed Rep. 2017 Apr;6(4):411-422. doi: 10.3892/br.2017.865. Epub 2017 Feb 22.
Calprotectin is a marker of inflammation and zonulin is a marker of intestinal permeability. Diets with lower carbohydrate content and higher contents of fat, fiber and protein, e.g., Okinawan-based diet, are considered to reduce inflammation and intestinal permeability. The aim of the present study was to evaluate calprotectin and zonulin levels in serum and feces after intervention with an Okinawan-based Nordic diet. Thirty patients (17 women) with type 2 diabetes, mean age 57.5±8.2 years, BMI 29.9±4.1 kg/m, were served the diet during 12 weeks, and were followed for another 16 weeks. Anthropometric and metabolic parameters were registered. Fasting levels of calprotectin and zonulin in serum and feces, and hormones in plasma, were measured by Luminex or ELISA before study start and after 12 and 28 weeks. Calprotectin in serum tended to be increased (P=0.074) after 12 weeks. Zonulin in serum and feces were elevated after 12 weeks (P=0.019 vs. P<0.001), and remained elevated in serum after 28 weeks (P=0.014). In contrast to baseline, there was a correlation between calprotectin and zonulin in serum and feces after dietary intervention (P=0.025 vs. P=0.079). Energy percentage of protein in breakfast correlated with serum calprotectin (P=0.008) and tended to correlate with serum zonulin (P=0.059). Calprotectin in serum tended to be elevated, and zonulin in serum and feces are elevated after introduction of an Okinawan-based Nordic diet. These biomarkers correlate with energy percentage of protein.
钙卫蛋白是炎症标志物，而闭合蛋白是肠道通透性标志物。碳水化合物含量较低、脂肪、纤维和蛋白质含量较高的饮食，如冲绳式饮食，被认为可减轻炎症和肠道通透性。本研究旨在评估采用基于冲绳式的北欧饮食进行干预后血清和粪便中的钙卫蛋白和闭合蛋白水平。30例2型糖尿病患者（17名女性），平均年龄57.5±8.2岁，体重指数29.9±4.1kg/m²，接受该饮食12周，并再随访16周。记录人体测量和代谢参数。在研究开始前以及12周和28周后，通过Luminex或酶联免疫吸附测定法测量血清和粪便中钙卫蛋白和闭合蛋白的空腹水平以及血浆中的激素水平。12周后血清中的钙卫蛋白有升高趋势（P=0.074）。12周后血清和粪便中的闭合蛋白升高（分别为P=0.019和P<0.001），28周后血清中仍保持升高（P=0.014）。与基线相比，饮食干预后血清和粪便中的钙卫蛋白和闭合蛋白之间存在相关性（分别为P=0.025和P=0.079）。早餐中蛋白质能量百分比与血清钙卫蛋白相关（P=0.008），并与血清闭合蛋白有相关趋势（P=0.059）。采用基于冲绳式的北欧饮食后，血清中的钙卫蛋白有升高趋势，血清和粪便中的闭合蛋白升高。这些生物标志物与蛋白质能量百分比相关。
